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EMAGE:19893

Dzip1l DAZ interacting protein 1-like ( MGI:1919757)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19893 EMAGE:19893 EMAGE:19893 EMAGE:19893 EMAGE:19893
"Pseudo-wholemount" of euxassay_006440. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006440_01 euxassay_006440_02 euxassay_006440_03 euxassay_006440_04
EMAGE:19893 EMAGE:19893 EMAGE:19893 EMAGE:19893 EMAGE:19893
euxassay_006440_05 euxassay_006440_06 euxassay_006440_07 euxassay_006440_08 euxassay_006440_09
EMAGE:19893 EMAGE:19893 EMAGE:19893 EMAGE:19893 EMAGE:19893
euxassay_006440_10 euxassay_006440_11 euxassay_006440_12 euxassay_006440_13 euxassay_006440_14
EMAGE:19893 EMAGE:19893 EMAGE:19893 EMAGE:19893 EMAGE:19893
euxassay_006440_15 euxassay_006440_16 euxassay_006440_17 euxassay_006440_18 euxassay_006440_19
EMAGE:19893 EMAGE:19893 EMAGE:19893 EMAGE:19893
euxassay_006440_20 euxassay_006440_21 euxassay_006440_22 euxassay_006440_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19893Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19893_wholemount_strong.wlz
19893_wholemount_moderate.wlz
19893_wholemount_weak.wlz
19893_wholemount_possible.wlz
19893_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19893_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 weak expression: see section 11 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35079
Entity Detected:Dzip1l, DAZ interacting protein 1-like ( MGI:1919757)
Sequence:sense strand is shown

>T35079
TAAAGTTCTCCCTAGGTCAGCGGCCAAGCCTGAGGACAACTGGGGCTGGTCTGATTCCGAGACCTCAGAG
GAGAGTGCCCAACCTCCTGGGAAGGGCTCAGGCGGGCTGGCTTCTTCAGGAACACTGGTGCAGTCAATTG
TCAAAAACCTTGAGAAGCAGCTTGAAACGCCAGCCAAGAAGCCTTCCGGAGGGGTCAATATGTTCTTAAG
GCCCAACGCTGCCCTCCAGAGGGCTTCCACACCAGCAAGGAAGTCTCAGCTTTCAGAAGATGAGAGTGAC
GTGGAGATCTCGTCTTTGGAAGATCTCTCCCAAGACCTGGGCCAGAAAGGAAAGCCAAAGCCTTTGTCCC
ACTCAAAGCTCCCAGAAAAGTTTGATGTCAGCCCTTGGAGCTCGGGGTCACGACCCAGGATTCCTGGCTG
GTGATTCTCTTATGTTTGCCAGAGGGCTTAGCCTTGTTTCTTGGCTGGTCTCACCCAAATGGAAGAGGCC
GTCAGGCCTCCCGCTCTCACTAGCAGCCAAGAAATCTTTCCCTTGAAGAGAAGCAGGGTAGGAGACAGAC
AGAACCTCTATCCACCCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 65630. Forward Primer - name:065630_F_cDNA_2610524A10Rik, sequence:TAAAGTTCTCCCTAGGTCAGCG; Reverse Primer - name:065630_N_SP6_cDNA_2610524A10Rik, sequence:GGGGGTGGATAGAGGTTCTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19890 same embryo
 EMAGE:19894 same embryo
 EMAGE:19891 same embryo
 EMAGE:19892 same embryo
 EurExpress:euxassay_006440 same experiment
 MGI:4824439 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS