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EMAGE:19898

Mfap3l microfibrillar-associated protein 3-like ( MGI:1918556)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19898 EMAGE:19898 EMAGE:19898 EMAGE:19898 EMAGE:19898
euxassay_009357_01 euxassay_009357_02 euxassay_009357_03 euxassay_009357_04 euxassay_009357_05
EMAGE:19898 EMAGE:19898 EMAGE:19898 EMAGE:19898 EMAGE:19898
euxassay_009357_06 euxassay_009357_07 euxassay_009357_08 euxassay_009357_09 euxassay_009357_10
EMAGE:19898 EMAGE:19898 EMAGE:19898 EMAGE:19898 EMAGE:19898
euxassay_009357_11 euxassay_009357_13 euxassay_009357_14 euxassay_009357_15 euxassay_009357_16
EMAGE:19898 EMAGE:19898 EMAGE:19898 EMAGE:19898 EMAGE:19898
euxassay_009357_17 euxassay_009357_18 euxassay_009357_19 euxassay_009357_20 euxassay_009357_21
EMAGE:19898 EMAGE:19898
euxassay_009357_22 euxassay_009357_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19898Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19898_wholemount_strong.wlz
19898_wholemount_moderate.wlz
19898_wholemount_weak.wlz
19898_wholemount_possible.wlz
19898_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19898_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 11 13 weak expression: see section 17
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 13 15
pons mantle layer
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 13 15 17
tegmentum
moderate moderate
regionalmoderate expression: see section 09 10 11
facial vii ganglion
weak weak
regionalweak expression: see section 04 19
trigeminal v ganglion
weak weak
regionalweak expression: see section 02 03 04 16 17 18 19 20 21
basal columns
moderate moderate
regionalmoderate expression: see section 11 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36628
Entity Detected:Mfap3l, microfibrillar-associated protein 3-like ( MGI:1918556)
Sequence:sense strand is shown

>T36628
TGGCATCTAACATCTATGGCACTATAAACAACACGGTGACACTGAGAGTCATCTTTACCTCTGGAGACAT
GGGCGTGTACTATATGGTGGTGTGCCTCGTGGCCTTCACCATAGTCATGATCCTCAACATCACCCGCCTG
TGTATGATGAGCAGTCACCTAAAGAAGACTGAGAAAGCCATCAATGAGTTCTTTAGGACAGAAGGTGCAG
AGAAGCTGCAGAAGGCTTTTGAGATTGCTAAACGCATCCCCATCATAACCTCAGCCAAAACTCTAGAACT
TGCCAAAGTGACTCAGTTCAAAACCATGGAATTTGCTAGGTACATTGAAGAGCTTGCCAGGAGCGTGCCC
CTGCCTCCCCTCATCATGAACTGCAGGACGATCATGGAGGAGATCATGGAAGTAGTCGGGCTGGAGGAGC
AGGGGCAGAATTTTGTGAGGCATACCCCAGAAGGCCAGGAAGCCCCAGATAGGGACGAGGTATACACCAT
CCCCAACTCACTGAAGCGAAGTGAGTCCCCCACCGCTGACTCGGATGCTTCGTCATTGCATGAGCAGCCT
CAGCAGATTGCCATCAAGGTTTCAGTTCACCCCCAGTCCAAAAGGGATCATGTGGATGACCAGGAGGGAG
GACACTTTGAGGTCAAAGATGAAGAGGAGACAGAACCATCAGAGGAACATTCCCCAGAGACTGCAGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75057. Forward Primer - name:075057_F_cDNA_Mfap3l, sequence:TGGCATCTAACATCTATGGCAC; Reverse Primer - name:075057_N_SP6_cDNA_Mfap3l, sequence:TCTGCAGTCTCTGGGGAATGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19899 same embryo
 EMAGE:19896 same embryo
 EMAGE:19897 same embryo
 EurExpress:euxassay_009357 same experiment
 MGI:4826175 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS