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EMAGE:199

Actc1 actin, alpha, cardiac muscle 1 ( MGI:87905)
TS14 (9 dpc)
in situ hybridisation

Data Images
EMAGE:199 EMAGE:199
Fig 3B of Sassoon et al., 1988 [PMID:3075543] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 3A of Sassoon et al., 1988 [PMID:3075543] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Corresponding bright and darkfield images are shown below of a mid-parasagital section showing the early heart-forming presumptive atrial (a) and ventricular (v) compartments. Arrows denote points at which cells that are hybridized with the cardiac actin probe border with cells that do not.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
heart
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1276342
Entity Detected:Actc1, actin, alpha, cardiac muscle 1 ( MGI:87905)
Sequence:sense strand is shown

>MGI:1276342
AGGCGACTGACACCCAGTGCCTGCCACCAGCGCCAGCCCAGCTGAATCCAGCCGCCCCTAGCACGGTGAG
TCCCAGCCTTGCTCCCTGCAGGACCTTGTCAGCACTGTGCTTTTGTGCTCTTGGATCC
nt 662 - nt 789 of M59866.1
Notes:The probe used in this study by Sassoon et al., 1988 [PMID:3075543] is described as a BamHI genomic fragment which includes the first non-coding exon (see Fig 1B therein). NB. Note that the target sequence for this probe is the reverse and complement of the sequence in the link above (the sequence in M59866 is the reverse strand).
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:9 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Sassoon et al., 1988 [PMID:3075543] . Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:3075543] Sassoon DA, Garner I, Buckingham M 1988 Transcripts of alpha-cardiac and alpha-skeletal actins are early markers for myogenesis in the mouse embryo. Development (104):155-64
Links:MGI:1343046 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI