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EMAGE:19910

A930001N09Rik RIKEN cDNA A930001N09 gene ( MGI:1924378)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19910 EMAGE:19910 EMAGE:19910 EMAGE:19910 EMAGE:19910
"Pseudo-wholemount" of euxassay_009331. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009331_01 euxassay_009331_02 euxassay_009331_03 euxassay_009331_04
EMAGE:19910 EMAGE:19910 EMAGE:19910 EMAGE:19910 EMAGE:19910
euxassay_009331_05 euxassay_009331_06 euxassay_009331_07 euxassay_009331_08 euxassay_009331_09
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euxassay_009331_15 euxassay_009331_16 euxassay_009331_17 euxassay_009331_18 euxassay_009331_19
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euxassay_009331_20 euxassay_009331_21 euxassay_009331_22 euxassay_009331_23 euxassay_009331_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19910Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19910_wholemount_strong.wlz
19910_wholemount_moderate.wlz
19910_wholemount_weak.wlz
19910_wholemount_possible.wlz
19910_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19910_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35843
Entity Detected:A930001N09Rik, RIKEN cDNA A930001N09 gene ( MGI:1924378)
Sequence:sense strand is shown

>T35843
GCTTACACCATCACAGCAAGAGAGGATTCTGAGGCCTTCTGAGTGGAATCGAGATACCTTGCCAAGTAAT
ATGTACCAGAAAAATGGCTTACATCATGGGAAATACGCAGTGAAGAAATCACGGAGAACTGATGTGGAAG
ACCTTACTCCAAACCCTAAAAAACTACTTCAGATTGGTAATGAGCTGCGCAAGCTGAATAAGGTGATCAG
TGACCTGACTCCAGTTAGTGAGCTTCCCTTAACAGCAAGGCCAAGGTCAAGGAAAGAAAAAAATAAGCTG
GCATCCAGAGCTTGTAGGCTAAAGAAGAAAGCCCAGTATGAAGCTAATAAAGTGAAGTTGTGGGGCCTCA
ACACTGAATATGACAATTTATTGTTTGTAATCAACTCCATCAAGCAAGACATTGTAAACCGAGTTCAGAA
TCCAAGAGAAGAGAGAGAACCCAGCATGGGGCAGAAGCTTGAAATCCTCATTAAAGATACACTGGGTCTC
CCAGTCGCTGGGCAAACCTCAGAATTTGTTAACCAAGTGTTAGGGAAGACTGCTGAAGGCAACCCCACTG
GAGGCCTTGTAGGACTAAGGATACCAGCATCAAAAGTGTAATCAGCCTCATTGGACCACTGGTCAGAAAT
GTCTGTTTTTGTCATGTTATCCATTGTAAATTTTCATTCTGTTTTGCATGTCAATTAGCATTATGTAAAC
ATTTATAATTAGGTTACATTGTTTTAAAAACAATAGCATAAGTGAAGCATGATCCAAAATACTTGATTAT
TGCATTTTCAGAGCATAAACCAGTGACCCTGCTGCTGGCATGAGAAAGAAGCTCACACATTAAGTAAATA
TGAGGTACAGATTGTAAACATTTGTTGAAGCAGAGTGTTTTGGGTGAGTGAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66105. Forward Primer - name:066105_F_cDNA_A930001N09Rik, sequence:GCTTACACCATCACAGCAAGAG; Reverse Primer - name:066105_N_SP6_cDNA_A930001N09Rik, sequence:TTCACTCACCCAAAACACTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19906 same embryo
 EMAGE:19911 same embryo
 EMAGE:19908 same embryo
 EMAGE:19907 same embryo
 EMAGE:19909 same embryo
 EurExpress:euxassay_009331 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS