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EMAGE:19919

Ubr3 ubiquitin protein ligase E3 component n-recognin 3 ( MGI:1861100)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19919 EMAGE:19919 EMAGE:19919 EMAGE:19919 EMAGE:19919
"Pseudo-wholemount" of euxassay_009294. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009294_01 euxassay_009294_02 euxassay_009294_03 euxassay_009294_04
EMAGE:19919 EMAGE:19919 EMAGE:19919 EMAGE:19919 EMAGE:19919
euxassay_009294_05 euxassay_009294_06 euxassay_009294_07 euxassay_009294_08 euxassay_009294_09
EMAGE:19919 EMAGE:19919 EMAGE:19919 EMAGE:19919 EMAGE:19919
euxassay_009294_10 euxassay_009294_11 euxassay_009294_12 euxassay_009294_13 euxassay_009294_14
EMAGE:19919 EMAGE:19919 EMAGE:19919 EMAGE:19919 EMAGE:19919
euxassay_009294_15 euxassay_009294_16 euxassay_009294_17 euxassay_009294_18 euxassay_009294_19
EMAGE:19919 EMAGE:19919 EMAGE:19919
euxassay_009294_20 euxassay_009294_21 euxassay_009294_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19919Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19919_wholemount_strong.wlz
19919_wholemount_moderate.wlz
19919_wholemount_weak.wlz
19919_wholemount_possible.wlz
19919_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19919_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 03 04 19
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 weak expression: see section 05 16
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 16 17 18 19 20 weak expression: see section 07 08
vestibulocochlear viii ganglion
weak weak
regionalweak expression: see section 05 06 16
dorsal root ganglion
weak weak
regionalweak expression: see section 05 06 07 08 09 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35801
Entity Detected:Ubr3, ubiquitin protein ligase E3 component n-recognin 3 ( MGI:1861100)
Sequence:sense strand is shown

>T35801
AGCGGGTTTTGTAGAAGACATCAGATTAAATCAAGTTCAAATATTCCCTGTGTCCCCAAAGACTTACTGA
TGATGTCTGAATTTGTTCTTCCAAGATTCATTTTTTGTCTTATTCAGTACTTAAGAGAAGGCTATAATGA
ACCAGCTGATGCACCATCAGAAAAAGATCTGAACAAAGTCCTTCAGCTTTTGGAGCCTCAGATTTCCTTT
TTAGAAGACCTGACTAAAATGGGAGGAGCCATGAGGTCTGTTCTTACTCAGGTGTTAACCAACCAGCAGA
ACTACAAAGACCTGACTGCGGGTCTTGGAGAAAATGCTTGTGCAAAGAAAAGTCACGAGAAGTACCTTAT
AGCTTTGAAGAGCTCCGGACTGACATATCCCGAGGATAAGCTGGTGTATGGGGTCCAGGAACCAGCTGCT
GGTACTAGCACTCTGGCAGCTCAAGGTTTTGCAGGTGCTACTGGAACACTGGGGCAGATAGACTCTTCAG
ATGAGGAGGACCAGGATGGGAGTCAAGGCTTGGGCAAGAGAAAGAGGGTGAAGCTAAGCAGTGGCACCAA
AGATCAATCCATAATGGATGTTTTGAAACATAAAAGTTTTTTAGAAGAACTATTATTTTGGACAATAAAG
TATGAGTTCCCACAGAAGATGGTAACTTTCTTGCTCAATATGCTCCCAGATCAAGAGTATAAGGTTGCTT
TTACAAAAACATTTGTTCAGCATTATGCTTTTATTATGAAAACACTGAAGAAAAGTCACGAATCAGATAC
AATGTCCAACAGAATTGTGCATATTAGTGTTCAGTTGTTCAGCAATGAGGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70442. Forward Primer - name:070442_F_cDNA_A130030D10Rik, sequence:AGCGGGTTTTGTAGAAGACATC; Reverse Primer - name:070442_N_SP6_cDNA_A130030D10Rik, sequence:TCCTCATTGCTGAACAACTGAAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19918 same embryo
 EurExpress:euxassay_009294 same experiment
 MGI:4829065 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS