EMAGE mouse gene expression spatial database

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EMAGE:19929

Myo10 myosin X ( MGI:107716)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009373. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009373_01	euxassay_009373_02	euxassay_009373_03	euxassay_009373_04

euxassay_009373_06	euxassay_009373_07	euxassay_009373_08	euxassay_009373_09	euxassay_009373_10

euxassay_009373_11	euxassay_009373_12	euxassay_009373_13	euxassay_009373_14	euxassay_009373_15

euxassay_009373_16	euxassay_009373_17	euxassay_009373_18	euxassay_009373_19	euxassay_009373_20

euxassay_009373_21	euxassay_009373_22	euxassay_009373_23	euxassay_009373_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19929_wholemount_strong.wlz

19929_wholemount_moderate.wlz

19929_wholemount_weak.wlz

19929_wholemount_possible.wlz

19929_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19929_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14 15 16

telencephalon mantle layer

moderate

single cell

moderate expression: see section 02 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon ventricular layer

strong

regional

strong expression: see section 02 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata alar plate mantle layer

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 moderate expression: see section 06

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13

rest of cerebellum mantle layer

moderate

single cell

moderate expression: see section 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16 17 18 moderate expression: see section 06

pons mantle layer

moderate

single cell

moderate expression: see section 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 moderate expression: see section 06

midbrain mantle layer

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain marginal layer

moderate

single cell

moderate expression: see section 19

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

trigeminal v ganglion

moderate

single cell

moderate expression: see section 06 07 08 09 18 19 20 21

spinal cord mantle layer

moderate

single cell

moderate expression: see section 07 08 09 10 11 12 13 14

spinal cord ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36658
Entity Detected:	Myo10, myosin X ( MGI:107716)
Sequence:	sense strand is shown >T36658 CCCTATGGGGACATAAATCTCAACCTGCTGAAAGACAAAGGCTACACGACCCTTCAGGACGAAGCCATCA AGATATTCAATTCTCTCCAACAACTGGAGTCCATGTCCGACCCCATCCCTATCATCCAGGGCATCCTGCA GACCGGGCACCACCTCCGGCCTCTGCGGGATGAACTGTATTGTCAGCTCATCAAACAGACCAACAAGGTC CCCCATCCCGGCAGCGTGGGCAACCTTTACAGCTGGCAGATCCTGACCTGCCTCAGCTGCACCTTCCTGC CTAGCCTCGGGATCCTCAAATACCTCAAGTTCCACCTGAAAAGGATACGGGAACAGTTCCCAGGAACCGA GATGGAGAAGTACGCCCTCTTTATCTACGAATCCCTTAAGAAAACCAAATGCAGAGAGTTTGTGCCTTCC CGAGATGAAATCGAAGCTCTGATCCACCGGCAGGAAATGACGTCCACAGTGTATTGCCATGGCGGAGGCT CCTGCAAGATCACCATCAACTCCCACACCACAGCCGGGGAGGTGGTGGAGAAGCTGATCCGAGGGCTCGC CATGGAGGACAGCAGGAACATGTTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 100374. Forward Primer - name:100374_F_cDNA_Myo10, sequence:CCCTATGGGGACATAAATCTCA; Reverse Primer - name:100374_N_SP6_cDNA_Myo10, sequence:AAACATGTTCCTGCTGTCCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19932 same embryo
	EMAGE:19931 same embryo
	EMAGE:19930 same embryo
	EurExpress:euxassay_009373 same experiment
	MGI:4826556 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source