EMAGE mouse gene expression spatial database

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EMAGE:19932

Myl1 myosin, light polypeptide 1 ( MGI:97269)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009372. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009372_01	euxassay_009372_02	euxassay_009372_03	euxassay_009372_04

euxassay_009372_05	euxassay_009372_06	euxassay_009372_07	euxassay_009372_08	euxassay_009372_09

euxassay_009372_10	euxassay_009372_11	euxassay_009372_12	euxassay_009372_13	euxassay_009372_14

euxassay_009372_15	euxassay_009372_16	euxassay_009372_17	euxassay_009372_18	euxassay_009372_19

euxassay_009372_20	euxassay_009372_21	euxassay_009372_22	euxassay_009372_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19932_wholemount_strong.wlz

19932_wholemount_moderate.wlz

19932_wholemount_weak.wlz

19932_wholemount_possible.wlz

19932_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19932_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 20 21 22 23

upper leg muscle

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

forearm rest of mesenchyme

strong

regional

strong expression: see section 01

hand

strong

regional

strong expression: see section 02 03 04 05

foot

strong

regional

strong expression: see section 10 11 22 23

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 22 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

eye skeletal muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 17 18 19 20 21 22 23

tongue muscle

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

tail paraxial mesenchyme

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36657
Entity Detected:	Myl1, myosin, light polypeptide 1 ( MGI:97269)
Sequence:	sense strand is shown >T36657 TCTGGAGGAGATCCTTCTTTTGTTAACCCATCCTTTTAATCAAAATGGCACCAAAGAAAGACGTGAAGAA GCCCGCTGCTGCGCCTGCCCCAGCCCCGGCCCCGGCCCCTGCCCCAGCCAAACCTAAGGAAGAAAAAATC GATCTGTCTGCTATTAAGATCGAGTTCTCTAAGGAGCAACAGGAGGACTTCAAGGAGGCATTTCTCCTGT TTGACAGAACAGGTGAATGCAAGATCACCTTAAGTCAGGTGGGAGACGTCCTCCGGGCTCTGGGCACCAA TCCCACCAATGCAGAGGTCAAGAAGGTTCTTGGGAACCCCAGCAATGAAGAGATGAATGCTAAGAAAATC GAGTTTGAACAGTTTCTGCCCATGATGCAAGCTATCTCCAACAACAAGGACCAGGGAGGTTATGAAGATT TCGTTGAGGGTCTGCGTGTCTTCGACAAGGAGGGCAATGGCACCGTCATGGGTGCTGAACTCCGCCATGT CCTCGCCACTCTGGGAGAGAAGATGAAGGAGGAAGAGGTAGAAGCGTTGCTGGCAGGCCAGGAGGACTCC AATGGCTGCATCAACTATGAAGCTTTTGTCAAACACATCATGTCTGTCTAAACGGAGTTTTCAAGCACGC AATGTTGGGGAAGACTGGCCAGTTCAAGAACACCTATGGCTAACTGTCAACACCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90231. Forward Primer - name:090231_F_cDNA_Myl1, sequence:TCTGGAGGAGATCCTTCTTTTG; Reverse Primer - name:090231_N_SP6_cDNA_Myl1, sequence:TGGTGTTGACAGTTAGCCATAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19929 same embryo
	EMAGE:19931 same embryo
	EMAGE:19930 same embryo
	EurExpress:euxassay_009372 same experiment
	MGI:4826547 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source