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EMAGE:19934

Myh9 myosin, heavy polypeptide 9, non-muscle ( MGI:107717)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19934 EMAGE:19934 EMAGE:19934 EMAGE:19934 EMAGE:19934
"Pseudo-wholemount" of euxassay_009371. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009371_01 euxassay_009371_02 euxassay_009371_04 euxassay_009371_05
EMAGE:19934 EMAGE:19934 EMAGE:19934 EMAGE:19934 EMAGE:19934
euxassay_009371_06 euxassay_009371_07 euxassay_009371_08 euxassay_009371_09 euxassay_009371_10
EMAGE:19934 EMAGE:19934 EMAGE:19934 EMAGE:19934 EMAGE:19934
euxassay_009371_12 euxassay_009371_13 euxassay_009371_14 euxassay_009371_15 euxassay_009371_16
EMAGE:19934 EMAGE:19934 EMAGE:19934 EMAGE:19934 EMAGE:19934
euxassay_009371_17 euxassay_009371_18 euxassay_009371_19 euxassay_009371_20 euxassay_009371_21
EMAGE:19934
euxassay_009371_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19934Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19934_wholemount_strong.wlz
19934_wholemount_moderate.wlz
19934_wholemount_weak.wlz
19934_wholemount_possible.wlz
19934_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19934_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 12 weak expression: see section 09 10 11
sublingual gland primordium
strong strong
regionalstrong expression: see section 08 14
submandibular gland primordium
strong strong
regionalstrong expression: see section 05 06 07 15 16 17
thyroid gland
weak weak
regionalweak expression: see section 09 13
vibrissa
moderate moderate
regionalmoderate expression: see section 04 05 19 20 21
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 21 22 weak expression: see section 01 02 18 19 20
cornea
weak weak
regionalweak expression: see section 01 02 21 22
naris
weak weak
regionalweak expression: see section 12 13
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 07 08 09 10 11 13 14 15 16 17
hindgut
weak weak
regionalweak expression: see section 09 10
midgut
weak weak
regionalweak expression: see section 08 09 10 12 13 14 15 16 17
lower jaw molar
moderate moderate
regionalmoderate expression: see section 06 17
upper jaw molar
moderate moderate
regionalmoderate expression: see section 05 06 17
metanephros
weak weak
regionalweak expression: see section 04 05 06 12 13 14 15
lung
weak weak
regionalweak expression: see section 01 02 04 05 06 07 08 11 12 13 14 15 16 17 18 19 20 21
trachea
moderate moderate
regionalmoderate expression: see section 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36656
Entity Detected:Myh9, myosin, heavy polypeptide 9, non-muscle ( MGI:107717)
Sequence:sense strand is shown

>T36656
CTGATACCAAAGAGTCCAGGCTGGCTCAGGCCGGATGACCCACAGGGTCTTGTCCTCCTTGCCTGAAAGC
ACGGGTGGTGGGCAAGAAGGGCGGCCATTGGAGTAGGCACAAGAGTTTTCTATGAATCTATTTTGTCTTC
AGATAAAGATTTTGATAGCTCAGGCCTCTAGTAGTGTTACCCTCCCCGACCTCGGCTGTCCCCGTCCCCC
GTCCCCCCTGCTGTTGGCAATCACACACGGTAACCTCATACCTGCCCTATGGCCCCCTTCCCTGGGCCCT
ATTGGTCCAGAAGGAGCCTCTGTCTGGGTGCAGAACATGGGGCACTCTGGGAATCCCCCCACTCCCTTCT
GGGCAGCACTGGTGCCTCTGCTCCTCCGACTGTAAACCGTCTCAAGTGCAATGCCCCTCCCCTCCCTTGC
CAAGGACAGACCGTCCTGGCACCGGGGCAAACCAGACAGGGCATCAGGGCCACTCTAGAAAGGCCAACAG
CCTTCCGGTGGCTTCTCCCAGCACTCTAGGGGACCAAATATATTTAATGGTTAAGGGACTTGCAGGGCCT
GGCAGCCAGAATATCCAAGGGCTGGAGCCCACTGTGCGCTCTGGTGCCTCTCCTAGGACTGGGGCCAAGG
GTGGTCGAGCTGTGCCACCCACTCTATAGCTTCAAGTCTGCCTTCCACAAGGATGCTTTTGAAAGAAAAA
AAAAGGTTTTATTTTTCCCTTCTTGTAGTAAGTGCTCTAGTTCTGGGTGTCTTCACTGCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91031. Forward Primer - name:091031_F_cDNA_Myh9, sequence:CTGATACCAAAGAGTCCAGGCT; Reverse Primer - name:091031_N_SP6_cDNA_Myh9, sequence:AGGCAGTGAAGACACCCAGAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19933 same embryo
 EMAGE:19936 same embryo
 EMAGE:19937 same embryo
 EMAGE:19938 same embryo
 EMAGE:19935 same embryo
 EurExpress:euxassay_009371 same experiment
 MGI:4826546 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS