EMAGE mouse gene expression spatial database

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EMAGE:19937

Mrc2 mannose receptor, C type 2 ( MGI:107818)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009360. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009360_01	euxassay_009360_02	euxassay_009360_03	euxassay_009360_04

euxassay_009360_05	euxassay_009360_06	euxassay_009360_07	euxassay_009360_08	euxassay_009360_09

euxassay_009360_10	euxassay_009360_11	euxassay_009360_12	euxassay_009360_13	euxassay_009360_14

euxassay_009360_15	euxassay_009360_16	euxassay_009360_17	euxassay_009360_18	euxassay_009360_19

euxassay_009360_20	euxassay_009360_21	euxassay_009360_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19937_wholemount_strong.wlz

19937_wholemount_moderate.wlz

19937_wholemount_weak.wlz

19937_wholemount_possible.wlz

19937_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19937_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

spotted

strong expression: see section 20 21 22 moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

diencephalon meninges

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon meninges

strong

spotted

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hindbrain meninges

strong

spotted

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain meninges

strong

spotted

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord meninges

strong

spotted

strong expression: see section 09 10 11 13 14

mandible

strong

spotted

strong expression: see section 07 08 09 10 14 15 16 17 18 19 20 moderate expression: see section 03 04 05 06

maxilla

strong

spotted

strong expression: see section 07 08 09 10 15 16 17 18 19 20 moderate expression: see section 05 06

temporal bone

moderate

spotted

moderate expression: see section 03

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36644
Entity Detected:	Mrc2, mannose receptor, C type 2 ( MGI:107818)
Sequence:	sense strand is shown >T36644 TGAACAAGAACCCCTGCTCTATACCAACTGGGCACCAGGAGAGCCCTCTGGCCCCAGCCCTGCTCCCAGT GGCACCAAGCCGACCAGCTGTGCGGTGATCCTGCACAGCCCCTCAGCCCACTTCACTGGCCGCTGGGATG ATCGGAGCTGCACAGAGGAGACGCATGGCTTCATCTGCCAGAAGGGCACAGACCCCTCGCTAAGCCCATC CCCAGCAGCAACACCCCCTGCCCCGGGCGCTGAGCTCTCCTATCTCAACCACACCTTCCGGCTGCTGCAG AAGCCACTGCGCTGGAAAGATGCTCTCCTGCTGTGTGAGAGCCGAAATGCCAGCCTGGCACACGTGCCCG ATCCCTACACACAAGCCTTCCTCACACAGGCTGCACGGGGGCTGCAAACACCACTGTGGATCGGGCTGGC CAGTGAGGAGGGCTCACGGAGGTATTCCTGGCTCTCAGAGGAGCCTCTGAATTATGTGAGCTGGCAAGAT GAGGAGCCCCAGCACTCGGGAGGCTGTGCCTACGTGGATGTGGATGGAACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98144. Forward Primer - name:098144_F_cDNA_Mrc2, sequence:TGAACAAGAACCCCTGCTCTAT; Reverse Primer - name:098144_N_SP6_cDNA_Mrc2, sequence:GGTTCCATCCACATCCACGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19933 same embryo
	EMAGE:19936 same embryo
	EMAGE:19938 same embryo
	EMAGE:19935 same embryo
	EMAGE:19934 same embryo
	EurExpress:euxassay_009360 same experiment
	MGI:4826437 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source