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EMAGE:19947

Pnmt phenylethanolamine-N-methyltransferase ( MGI:97724)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19947 EMAGE:19947 EMAGE:19947 EMAGE:19947 EMAGE:19947
"Pseudo-wholemount" of euxassay_009415. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009415_01 euxassay_009415_02 euxassay_009415_03 euxassay_009415_04
EMAGE:19947 EMAGE:19947 EMAGE:19947 EMAGE:19947 EMAGE:19947
euxassay_009415_05 euxassay_009415_06 euxassay_009415_07 euxassay_009415_08 euxassay_009415_09
EMAGE:19947 EMAGE:19947 EMAGE:19947 EMAGE:19947 EMAGE:19947
euxassay_009415_10 euxassay_009415_11 euxassay_009415_12 euxassay_009415_13 euxassay_009415_14
EMAGE:19947 EMAGE:19947 EMAGE:19947 EMAGE:19947 EMAGE:19947
euxassay_009415_15 euxassay_009415_16 euxassay_009415_17 euxassay_009415_18 euxassay_009415_19
EMAGE:19947 EMAGE:19947
euxassay_009415_20 euxassay_009415_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19947Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19947_wholemount_strong.wlz
19947_wholemount_moderate.wlz
19947_wholemount_weak.wlz
19947_wholemount_possible.wlz
19947_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19947_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
adrenal medulla
strong strong
regionalstrong expression: see section 15 moderate expression: see section 08 16
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 08 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36812
Entity Detected:Pnmt, phenylethanolamine-N-methyltransferase ( MGI:97724)
Sequence:sense strand is shown

>T36812
CTATCTCCGCAACAACTACGCGCCTCCTCGTGGAGACCTGAGCAACCCTGATGGCGTCGGGCCTTGGAAG
CTGCGCTGCATGGCACAAGTCTTTGCTACCGGTGAGGTGTCGGGACGGGTTCTCATTGATATTGGCTCCG
GCCCCACCATATATCAGCTGCTCAGTGCCTGTGCCCACTTTGAGGACATCACCATGACAGACTTCTTGGA
AGTCAACCGTCAGGAGCTGGGACTCTGGCTGCGAGAGGAGCCAGGAGCCTTTGACTGGAGTGTGTATAGT
CAGCATGCCTGCCTCATTGAGGACAAGGGTGAGTCCTGGCAGGAGAAAGAACGCCAGCTTCGAGCGAGGG
TGAAGCGAGTCCTGCCTATCGATGTGCACAAGCCCCAGCCCCTGGGAACTCCCAGTCTGGTCCCTCTGCC
TGCCGACGCCTTGGTCTCTGCCTTCTGCCTGGAGGCTGTGAGCCCAGATCTTACTAGCTTCCAGCGGGCT
TTGCATCACATCACCACACTGCTGAGGCCCGGGGGTCATCTCCTCCTCATTGGGGCCCTGGAGGAGTCCT
GGTACCTTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98364. Forward Primer - name:098364_F_cDNA_Pnmt, sequence:CTATCTCCGCAACAACTACGC; Reverse Primer - name:098364_N_SP6_cDNA_Pnmt, sequence:GCAAGGTACCAGGACTCCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19948 same embryo
 EMAGE:19951 same embryo
 EMAGE:19946 same embryo
 EMAGE:19950 same embryo
 EMAGE:19949 same embryo
 EurExpress:euxassay_009415 same experiment
 MGI:4827301 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS