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EMAGE:19961

Neto2 neuropilin (NRP) and tolloid (TLL)-like 2 ( MGI:1921763)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19961 EMAGE:19961 EMAGE:19961 EMAGE:19961 EMAGE:19961
"Pseudo-wholemount" of euxassay_009406. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009406_01 euxassay_009406_02 euxassay_009406_03 euxassay_009406_04
EMAGE:19961 EMAGE:19961 EMAGE:19961 EMAGE:19961 EMAGE:19961
euxassay_009406_05 euxassay_009406_06 euxassay_009406_07 euxassay_009406_08 euxassay_009406_09
EMAGE:19961 EMAGE:19961 EMAGE:19961 EMAGE:19961 EMAGE:19961
euxassay_009406_10 euxassay_009406_11 euxassay_009406_12 euxassay_009406_13 euxassay_009406_14
EMAGE:19961 EMAGE:19961 EMAGE:19961 EMAGE:19961 EMAGE:19961
euxassay_009406_15 euxassay_009406_16 euxassay_009406_17 euxassay_009406_18 euxassay_009406_19
EMAGE:19961
euxassay_009406_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19961Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19961_wholemount_strong.wlz
19961_wholemount_moderate.wlz
19961_wholemount_weak.wlz
19961_wholemount_possible.wlz
19961_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19961_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hypothalamus mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 12 13
thalamus mantle layer
weak weak
regionalweak expression: see section 07 08 09 10 12 15
cerebral cortex marginal layer
weak weak
regionalweak expression: see section 03 04 05 06 07 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36678
Entity Detected:Neto2, neuropilin (NRP) and tolloid (TLL)-like 2 ( MGI:1921763)
Sequence:sense strand is shown

>T36678
CCACCACTCCAACACCATAGTAAATACTTAAATTGATAGCTATGATACCATAGTGAAATAAAAATTAACA
ATTTTATAAATTATATCCACTATAAAAATAGTTGTCATATTCATCATTAATTTTAAGATTATAGTTTATA
GTATGCCTTCATACATCATGATCACTTGTAGGTGTATTTTTATTTTTAACACTATTCTATTTTAAAGGAA
TTTGAAGGGATTTACAAAACAGTTGTTTGTACAAAATACTGAACATTCCAACCATGTAAATTTGCATGAT
CTCATGCATCAGCAAGAGAAACTCGGGTGATCATTTGGTTCTAAATTCTCTAACTAGAAAGTTGATCCTT
TTCACAGTAGAAAACACTTATATTCAGAGGCTAAAGTGAAAGCCATAAACTTAAGCAGTAACAAATATGA
AGTTAAGCTGTCTTATGTGAGTTATTATACCCTACAAAGTACCTGTGTTAAACACACCCTTCAGTGAAAA
ATGCAAAGCCAGAGGATAGTAAAAATCTCCAGTGCAAGAAAGTACTAAGTACAAACTATATGATACCAAC
ACATTGCTGTCGTTCATCCCAAGTTCACTACAAGCTGTCAGCTCCCAAGTAGCCACAGACTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81448. Forward Primer - name:081448_F_cDNA_Neto2, sequence:CCACCACTCCAACACCATAGTA; Reverse Primer - name:081448_N_SP6_cDNA_Neto2, sequence:GCAGTCTGTGGCTACTTGGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19966 same embryo
 EMAGE:19965 same embryo
 EMAGE:19964 same embryo
 EMAGE:19962 same embryo
 EMAGE:19963 same embryo
 EurExpress:euxassay_009406 same experiment
 MGI:4826669 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS