EMAGE mouse gene expression spatial database

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EMAGE:19997

St8sia6 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 6 ( MGI:2386797)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009481. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009481_01	euxassay_009481_02	euxassay_009481_03	euxassay_009481_04

euxassay_009481_05	euxassay_009481_06	euxassay_009481_07	euxassay_009481_08	euxassay_009481_09

euxassay_009481_10	euxassay_009481_11	euxassay_009481_12	euxassay_009481_13	euxassay_009481_14

euxassay_009481_15	euxassay_009481_16	euxassay_009481_17	euxassay_009481_18	euxassay_009481_19

euxassay_009481_20	euxassay_009481_21	euxassay_009481_22	euxassay_009481_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19997_wholemount_strong.wlz

19997_wholemount_moderate.wlz

19997_wholemount_weak.wlz

19997_wholemount_possible.wlz

19997_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19997_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37020
Entity Detected:	St8sia6, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 6 ( MGI:2386797)
Sequence:	sense strand is shown >T37020 GAAGAAGGCACAGAGAAAGCATGAATTACAAAGGCGCTCTCCCACTTGTCTAGACCAAAGCCACCCGCCC CCACTCACTTTGCAGCCTCCACGAGTCACTCATTCTCACCTTCAACGTTCTTTCTCTGAGAATAGAGACC AAAACATCAGACTTGGATAAGTAAAATGAGATAATTTTTCAAATCATCATAGAATTTGATTTGAGCCAGG GTCTCTCAGAATGCTTCCTTGTTCCTATCCATGATAGCCATTCCCACCTTTATCAGAGTGGTAATGAAAC TGTGCAATTGTGCCAAAGACCCTTTCTGAAGAGAATGTCTGAATCATGCGCCGAGTTTTTACACACAGCT CTTCCTTTATAAATAAATCCTTCCCATTCTCCCTCCTAGTAGAGTACAGAAACAAAATACCCTTGATGAT TCAGGAAGAAAAGTCTTTTTTACTTAGCAATGTGCCTGCTTCTGATTCAGTTCGCTTGTGACATTAAGCT GGGTTGGGGTTTTGGTTGGATTTGGGGCGTTTCTTCACTTCTTTTGTCTATATTTTCCTTACCTTTATCA GTTTGTATTCGAGCTTCCTGCTTTGGGATTCTGCAATTCTCTCTCCCACTGACAGGATCAACTCAATGAC ATAAAGTAGTTCAAACATCCATTGCTTCTCACATGTTTTATCCATAAAGTTACTCATCTGATTTTATTTA AAATAGTGAACATCTACTTGATATCAGACCCGAGGACCATCCTCCATTGGAGAATATGAAGATATTGTCA CTGGCAGAAAAGCAGGTGTGTGCCATTAATTGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101864. Forward Primer - name:101864_F_cDNA_Siat8f, sequence:GAAGAAGGCACAGAGAAAGCAT; Reverse Primer - name:101864_N_SP6_cDNA_Siat8f, sequence:TCAATTAATGGCACACACCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19993 same embryo
	EMAGE:19996 same embryo
	EMAGE:19998 same embryo
	EMAGE:19994 same embryo
	EMAGE:19995 same embryo
	EurExpress:euxassay_009481 same experiment
	MGI:4828471 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source