EMAGE mouse gene expression spatial database

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EMAGE:19998

Shc3 src homology 2 domain-containing transforming protein C3 ( MGI:106179)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009466. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009466_01	euxassay_009466_02	euxassay_009466_03	euxassay_009466_04

euxassay_009466_05	euxassay_009466_06	euxassay_009466_07	euxassay_009466_08	euxassay_009466_09

euxassay_009466_10	euxassay_009466_11	euxassay_009466_12	euxassay_009466_13	euxassay_009466_14

euxassay_009466_15	euxassay_009466_16	euxassay_009466_17	euxassay_009466_18	euxassay_009466_19

euxassay_009466_20	euxassay_009466_21	euxassay_009466_22	euxassay_009466_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19998_wholemount_strong.wlz

19998_wholemount_moderate.wlz

19998_wholemount_weak.wlz

19998_wholemount_possible.wlz

19998_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19998_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15

olfactory cortex ventricular layer

strong

regional

strong expression: see section 11 12 16 17

telencephalon ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 weak expression: see section 23

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 moderate expression: see section 20

pons ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 13 14 15 16 18

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

ventral grey horn

moderate

regional

moderate expression: see section 10 12 14

spinal cord ventricular layer

moderate

regional

moderate expression: see section 10 11 12

cochlear duct

moderate

regional

moderate expression: see section 04 05 06 07 08 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37018
Entity Detected:	Shc3, src homology 2 domain-containing transforming protein C3 ( MGI:106179)
Sequence:	sense strand is shown >T37018 CTATGTCGCTAAGGACCCTGTCAATCGCAGAGCTTGCCACATTCTGGAATGCTGTGACGGGCTAGCCCAA GATGTCATTGGCTCCATCGGACAAGCCTTTGAACTCCGGTTCAAACAGTATTTGCAGTGCCCTTCCAAGG TTCCTGCCCTCCAGGACCGAATGCAGAGTCTGGATGAGCCATGGACTGAAGAAGAGGGAGATGGCCCTGA TCACCCATACTACAACAGCGTTCCCACCAAGATGCCTCCCCCAGGGGGGTTTCTGGATGCTCGATTGAAA GGCAGACCCCACGCTCCTGAGGCAGCCCAGTTTGCAGGAAAAGAGCAAACTTATTACCAGGGAAGACACT TAGGAGATACATTTGGTGAAGACTGGCAGCGAGCACCCACCAGGCAAGGCTCCTTGGACATCTATAGCAC AGCAGAAGGGAAAACTCACATGGTTCCTGTAGGAGAAACACCAACCTATGTCAACACCCAGCCAGTCCCA CCACAGGTGTGGCCAGCAGCAACCAGCAGCACTGAGAGCAGCCCACGGAAGGACCTCTTTGACATGAAGC CCTTTGAAGATGCCCTGAGAAACCAGCCCCTGGGCCCCATGTTGAGCAAAGCCGCGTCTGTGGAGTGCAT CAGCCCGGTCACACCCAGAGCCCCGGATGCCAGGATGCTGGAGGAGCTTAACGCTGAGCCCTGGTACCAA GGAGAGATGAGCAGGAAGGAGGCAGAGGCCCTGCTACGGGAAGATGGAGACTTCCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74102. Forward Primer - name:074102_F_cDNA_Shc3, sequence:CTATGTCGCTAAGGACCCTGTC; Reverse Primer - name:074102_N_SP6_cDNA_Shc3, sequence:AGGAAGTCTCCATCTTCCCGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19993 same embryo
	EMAGE:19996 same embryo
	EMAGE:19994 same embryo
	EMAGE:19995 same embryo
	EMAGE:19997 same embryo
	EurExpress:euxassay_009466 same experiment
	MGI:4828050 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source