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EMAGE:20007

Slc29a3 solute carrier family 29 (nucleoside transporters), member 3 ( MGI:1918529)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20007 EMAGE:20007 EMAGE:20007 EMAGE:20007 EMAGE:20007
"Pseudo-wholemount" of euxassay_009475. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009475_01 euxassay_009475_02 euxassay_009475_03 euxassay_009475_04
EMAGE:20007 EMAGE:20007 EMAGE:20007 EMAGE:20007 EMAGE:20007
euxassay_009475_05 euxassay_009475_06 euxassay_009475_07 euxassay_009475_08 euxassay_009475_09
EMAGE:20007 EMAGE:20007 EMAGE:20007 EMAGE:20007 EMAGE:20007
euxassay_009475_10 euxassay_009475_11 euxassay_009475_12 euxassay_009475_13 euxassay_009475_14
EMAGE:20007 EMAGE:20007 EMAGE:20007 EMAGE:20007 EMAGE:20007
euxassay_009475_15 euxassay_009475_16 euxassay_009475_17 euxassay_009475_18 euxassay_009475_19
EMAGE:20007 EMAGE:20007 EMAGE:20007 EMAGE:20007 EMAGE:20007
euxassay_009475_20 euxassay_009475_21 euxassay_009475_22 euxassay_009475_23 euxassay_009475_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20007Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20007_wholemount_strong.wlz
20007_wholemount_moderate.wlz
20007_wholemount_weak.wlz
20007_wholemount_possible.wlz
20007_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20007_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37031
Entity Detected:Slc29a3, solute carrier family 29 (nucleoside transporters), member 3 ( MGI:1918529)
Sequence:sense strand is shown

>T37031
GGACATCTACCCAGTGCTTTTCACCTGCCTCCTGGGGCTCAGCAACGGCTACCTCAGCACGCTGGTGCTC
ATCTATGGGCCCAAGATTGTGCCCCGGGAGCTGGCTGAGGCCACCAGTGTTGTGATGTTGTTCTATATGT
CTGTGGGCTTGATGCTGGGCTCAGCCTGCGCGGCCCTGCTTGAACACTTTATCTAGGAGGGGGCGGCAAG
GATGTGGGTACTGTGTGAGTGTGTGAGGCTTGGGTCCTTAGGAGATGGACAGGGTAAGCCAAGGTCTCGA
GGTGTTAAGCAAGGGGTTGACATTTTGTTTGATGCAGAGCAGAGCCCACTCAGACTGCCTTTCTCTCAGA
CTGCTTGTGAGCCACATCCTTGCACATCTTGGGCAAGGAGACATTCCAAACACAGCCCAGCCTGGCTCAT
ACCTGTAATCTCAGCATTCAAGAGAATGAAGCTGGAGGCCCACTGTGAGTTCAAGGCCAGCCTGGTTACA
TAGTGAAACCTTTTCTCAAAAAATAAGCTAAAAACAAAAACCCTGAGACATTCCAAACACTTCCAGAGAA
CACTCCAGAGCCGACTGAAGGGCAGGGTGAGGTTGTAGCCAGGATCTAGCCCAGTTGGTAGGATGCTTGT
TTAGCCTCCATAGAGCCCCAGCTCCTATCCCCAGCCCCGAATAAGCTGGGCATGTTAGTGAAAGCCTGTA
ATCCTAGCTCTTGAAAGGTGGAAGCAGCAGGGTCAGAAGTTCAGGTCATCCTCAGCTATTTAGGGAGTTT
GAGGCTAACCTGGGCTACAGGAGACACTGTCTCAAAAAAAAAAAAGACGCAATTGTAAAGAGTGGGGTAT
GTTACAGTCATAGCTCATAAGTAGCCCATCTCTAATTTGTATTGTCACAAGGCCAGAAGCCAGCCTCACT
CTTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 71247. Forward Primer - name:071247_F_cDNA_Slc29a3, sequence:GGACATCTACCCAGTGCTTTTC; Reverse Primer - name:071247_N_SP6_cDNA_Slc29a3, sequence:GAAAGAGTGAGGCTGGCTTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20008 same embryo
 EMAGE:20005 same embryo
 EMAGE:20010 same embryo
 EMAGE:20009 same embryo
 EMAGE:20006 same embryo
 EurExpress:euxassay_009475 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS