EMAGE mouse gene expression spatial database

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EMAGE:20020

Islr immunoglobulin superfamily containing leucine-rich repeat ( MGI:1349645)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002017. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002017_01	euxassay_002017_02	euxassay_002017_03	euxassay_002017_04

euxassay_002017_05	euxassay_002017_06	euxassay_002017_07	euxassay_002017_08	euxassay_002017_09

euxassay_002017_10	euxassay_002017_11	euxassay_002017_12	euxassay_002017_13	euxassay_002017_14

euxassay_002017_15	euxassay_002017_16	euxassay_002017_17	euxassay_002017_18	euxassay_002017_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20020_wholemount_strong.wlz

20020_wholemount_moderate.wlz

20020_wholemount_weak.wlz

20020_wholemount_possible.wlz

20020_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20020_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

limb

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 10 11 17 18 19

mesenchyme

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19

vertebral axis musculature

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

gland

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

alimentary system

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14

integumental system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

diencephalon meninges

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 10 11 12

telencephalon meninges

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

hindbrain meninges

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

midbrain meninges

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14

spinal cord meninges

strong

homogeneous

strong expression: see section 06 07 08 09 10 11

urinary system

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

reproductive system

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

respiratory system

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3444
Entity Detected:	Islr, immunoglobulin superfamily containing leucine-rich repeat ( MGI:1349645)
Sequence:	sense strand is shown >T3444 TGGCCTCGAGCCAGATTCGGACGAGGGCGACCTGCACAACCTCAGCGCTCTGCAGCTGCTCAAGATGGAC AGCAACGAGTTAGCCTTCATCCCTCGGGACGCCTTTAGCAGCCTCAGCGCCCTGCGTTCCCTGCAGCTCA ACCACAACCGCCTGCACGCATTGGCCGAGGGCACCTTCGTGCCGCTCACCGCGCTGTCCCACTTGCAGAT CAATGACAACCCTTTTGACTGCACCTGTGGCATCGTGTGGTTCAAGACATGGGCCCTGGCCTCAGCGGTG TCTATTCCAGAACAGGACAACATTGCCTGTACTACACCCCACGTCCTGAAGGGTATCCCACTAGGCCGCC TGCCACCCCTGCCCTGCTCAGCTCCCTCAGTGCAACTAAGCTACCAGCCCAGCCAAGATGGAGCAGAGCT ACGGCCTGGCTTCGTGCTGGCACTCCACTGTGACGTGGATGGACAGCCAGTCCCCCAGCTCCATTGGCAC ATTCACACCCCGGGCGGCACGGTGGAGATCGCCAGTCCTAATGTAGGCACTGATGGACGTGCCCTGCC
Notes:	The probe template was PCR amplified from IMAGE:3026058 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3026058 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20018 same embryo
	EMAGE:20017 same embryo
	EMAGE:20021 same embryo
	EMAGE:20019 same embryo
	EurExpress:euxassay_002017 same experiment
	MGI:4825631 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source