EMAGE mouse gene expression spatial database

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EMAGE:20022

Aldh1l1 aldehyde dehydrogenase 1 family, member L1 ( MGI:1340024)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009095. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009095_01	euxassay_009095_02	euxassay_009095_03	euxassay_009095_04

euxassay_009095_05	euxassay_009095_06	euxassay_009095_07	euxassay_009095_08	euxassay_009095_09

euxassay_009095_10	euxassay_009095_11	euxassay_009095_12	euxassay_009095_13	euxassay_009095_14

euxassay_009095_15	euxassay_009095_16	euxassay_009095_17	euxassay_009095_18	euxassay_009095_19

euxassay_009095_20	euxassay_009095_21	euxassay_009095_22	euxassay_009095_23	euxassay_009095_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20022_wholemount_strong.wlz

20022_wholemount_moderate.wlz

20022_wholemount_weak.wlz

20022_wholemount_possible.wlz

20022_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20022_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 08 09 10 16 17

hypothalamus ventricular layer

strong

regional

strong expression: see section 11 12 13 14

diencephalon lateral wall mantle layer

strong

single cell

strong expression: see section 07 08 13

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14

telencephalon mantle layer

strong

single cell

strong expression: see section 02 03 04 05 07 08 17 18 19 20 21 22 23

olfactory cortex ventricular layer

strong

regional

strong expression: see section 10 11 12 16 17 19 20 21 22 not examined expression: see section 23

telencephalon ventricular layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 16 17 18 23

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 06 07 08 09 12 13 14 15 16

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons mantle layer

strong

single cell

strong expression: see section 07 08 12 13 14 15 16 17

pons ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain mantle layer

strong

single cell

strong expression: see section 05 06 07 08 09 13 14 15 16

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15

ventral grey horn

strong

single cell

strong expression: see section 09 10 11 12 13 14

spinal cord ventricular layer

strong

single cell

strong expression: see section 10 11 12 13

testis

moderate

regional

moderate expression: see section 05 06 07 08 19 weak expression: see section 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1213
Entity Detected:	Aldh1l1, aldehyde dehydrogenase 1 family, member L1 ( MGI:1340024)
Sequence:	sense strand is shown >T1213 CCTCGAGACTGTTGGCCTACTGGCTACGTGGAANAGGCAGTGAAGAAACTGACTCTCCAAATGCCCTACC AGCTCTTCATAGGCGGCGAGTTTGTGGATGCTGAGGGCGCGAAGACCTACAGTACCATAAACCCAACGGA TGGAAGTGTCATCTGCCAGGTGTCTCTAGCTCAGGTGAGTGATGTTGACAAGGCGGTGGCAGCAGCGAAG GAGGCCTTTGAGAATGGACTGTGGGGAAAGATAAATGCGCGTGACCGGGGCCGGCTCCTGTACAGGTTGG CGGACCTCATGGAGCAGCACCAGGAAGAGCTAGCCACCATTGAGGCCCTGGACGCAGGTGCCGTCTACAC GCTGGCCCTGAAGACGCATGTGGGCATGTCCATCCAGACCTTCCGATACTTCGCTGGCTGGTGTGATAAG ATCCAGGGTGCCACCATCCCCATCAACCAGGCTAGACCCAACCGCAACCTGACCTTGACCAAGAAGGAAC CTGTTGGGGTCTGTGGTATTGTCATCCCCTGGAACTATCCCTTAATGATGCTGTCCTGGAAGACAGCAGC CTGCCTGGCTGCCGGGAACACCGTG
Notes:	The probe template was PCR amplified from IMAGE:2192278 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2192278 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20026 same embryo
	EMAGE:20024 same embryo
	EMAGE:20023 same embryo
	EMAGE:20025 same embryo
	EurExpress:euxassay_009095 same experiment
	MGI:4823082 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source