EMAGE mouse gene expression spatial database

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EMAGE:20023

Xrcc5 X-ray repair complementing defective repair in Chinese hamster cells 5 ( MGI:104517)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009101. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009101_01	euxassay_009101_02	euxassay_009101_03	euxassay_009101_04

euxassay_009101_05	euxassay_009101_06	euxassay_009101_07	euxassay_009101_08	euxassay_009101_09

euxassay_009101_10	euxassay_009101_11	euxassay_009101_12	euxassay_009101_13	euxassay_009101_14

euxassay_009101_15	euxassay_009101_16	euxassay_009101_17	euxassay_009101_18	euxassay_009101_19

euxassay_009101_20	euxassay_009101_21	euxassay_009101_22	euxassay_009101_23	euxassay_009101_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20023_wholemount_strong.wlz

20023_wholemount_moderate.wlz

20023_wholemount_weak.wlz

20023_wholemount_possible.wlz

20023_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20023_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1211
Entity Detected:	Xrcc5, X-ray repair complementing defective repair in Chinese hamster cells 5 ( MGI:104517)
Sequence:	sense strand is shown >T1211 CCTCNAGCCTGTTGGCCTACTGGNATATAAAACTGAGAAAGAAGAAGATCACATCAGCATCTCCAGCCTG GCAGAAGGGAACATCACCAAGGTTGGAAGTGTGAATCCTGTTGAAAACTTCCGTTTCCTAGTAAGACAGA AGATTGCCAGCTTTGAGGAAGCGAGTCTCCAGTTAATAAGTCACATCGAACAATTTTTGGATACCAATGA AACACTGTATTTTATGAAAAGTATGGACTGCATCAAAGCTTTCCGGGAGGAGGCCATTCAGTTTTCAGAA GAACAGCGCTTCAACAGCTTCCTGGAAGCCCTTCGAGAGAAAGTGGAAATTAAGCAATTAAATCATTTCT GGGAAATTGTTGTTCAGGATGGAGTTACTCTGATCACCAAAGACGAAGGCCCAGGAAGCTCTATCACAGC TGAGGAAGCCACAAAGTTTCTGGCCCCCAAAGACAAAGCAAAAGAAGATACAACGGGACCTGAAGAAGCT GGTGATGTGGATGATTTACTGGACATGATATAGATCATGGATGTGTGGGGAATCCAAGAGTGCCAGCTCT GTGCTG
Notes:	The probe template was PCR amplified from IMAGE:2192218 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2192218 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20022 same embryo
	EMAGE:20026 same embryo
	EMAGE:20024 same embryo
	EMAGE:20025 same embryo
	EurExpress:euxassay_009101 same experiment
	MGI:4829246 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source