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EMAGE:20041

Slc5a5 solute carrier family 5 (sodium iodide symporter), member 5 ( MGI:2149330)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20041 EMAGE:20041 EMAGE:20041 EMAGE:20041 EMAGE:20041
"Pseudo-wholemount" of euxassay_012146. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012146_01 euxassay_012146_02 euxassay_012146_03 euxassay_012146_04
EMAGE:20041 EMAGE:20041 EMAGE:20041 EMAGE:20041 EMAGE:20041
euxassay_012146_05 euxassay_012146_06 euxassay_012146_07 euxassay_012146_08 euxassay_012146_09
EMAGE:20041 EMAGE:20041 EMAGE:20041 EMAGE:20041 EMAGE:20041
euxassay_012146_10 euxassay_012146_11 euxassay_012146_12 euxassay_012146_13 euxassay_012146_14
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euxassay_012146_15 euxassay_012146_16 euxassay_012146_17 euxassay_012146_18 euxassay_012146_19
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euxassay_012146_20 euxassay_012146_21 euxassay_012146_22 euxassay_012146_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20041Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20041_wholemount_strong.wlz
20041_wholemount_moderate.wlz
20041_wholemount_weak.wlz
20041_wholemount_possible.wlz
20041_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20041_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
stomach
moderate moderate
regionalmoderate expression: see section 07 08 weak expression: see section 05 06
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37039
Entity Detected:Slc5a5, solute carrier family 5 (sodium iodide symporter), member 5 ( MGI:2149330)
Sequence:sense strand is shown

>T37039
CAGGAATCATCTGCACCTTGTACACGACCGTGGGTGGTATGAAGGCCGTGGTCTGGACAGATGTGTTCCA
GGTTGTGGTAATGCTCGTCGGCTTCTGGGTGATCCTGGCTCGAGGTGTCATGCTCATGGGGGGCCCCTGG
AACGTGCTCAGTCTCGCTCAAAACCATTCCCGGATCAACCTGATGGACTTTGACCCTGACCCTCGGAGCC
GCTACACCTTCTGGACATTTGTAGTAGGTGGCTCCCTGGTGTGGCTCTCCATGTATGGTGTGAACCAAGC
CCAGGTGCAGCGTTATGTGGCCTGTCACACGGAGAGAAAGGCCAAGCTTGCCCTGCTTGTCAACCAGCTT
GGCCTCTTCCTGATTGTGGCTAGTGCAGCTTGCTGTGGAATTGTCATGTTTGTCTACTACAAGGACTGCG
ACCCCCTCCTCACAGGCCGCATCGCAGCCCCCGATCAGTACATGCCATTGCTCGTGTTGGACATTTTTGA
GGATCTGCCCGGAGTCCCCGGGCTCTTCCTGGCCTGTGCCTACAGTGGCACCCTCAGCACCGCGTCCACC
AGTATCAACGCTATGGCAGCTGTAACTGTGGAAGACCTCATCAAGCCCAGGATGCCTAGCCTGGCACCCC
GGAAGCTGGTTTTCATCTCTAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92109. Forward Primer - name:092109_F_cDNA_Slc5a5, sequence:CAGGAATCATCTGCACCTTGTA; Reverse Primer - name:092109_N_SP6_cDNA_Slc5a5, sequence:TTAGAGATGAAAACCAGCTTCCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20040 same embryo
 EMAGE:20037 same embryo
 EMAGE:20042 same embryo
 EMAGE:20039 same embryo
 EMAGE:20038 same embryo
 EurExpress:euxassay_012146 same experiment
 MGI:4828253 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS