EMAGE mouse gene expression spatial database

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EMAGE:20042

Slc4a11 solute carrier family 4, sodium bicarbonate transporter-like, member 11 ( MGI:2138987)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012145. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012145_01	euxassay_012145_02	euxassay_012145_03	euxassay_012145_04

euxassay_012145_05	euxassay_012145_06	euxassay_012145_07	euxassay_012145_08	euxassay_012145_09

euxassay_012145_10	euxassay_012145_11	euxassay_012145_12	euxassay_012145_13	euxassay_012145_14

euxassay_012145_15	euxassay_012145_16	euxassay_012145_17	euxassay_012145_18	euxassay_012145_19

euxassay_012145_20	euxassay_012145_21	euxassay_012145_22	euxassay_012145_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20042_wholemount_strong.wlz

20042_wholemount_moderate.wlz

20042_wholemount_weak.wlz

20042_wholemount_possible.wlz

20042_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20042_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vomeronasal organ

weak

regional

weak expression: see section 07 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37038
Entity Detected:	Slc4a11, solute carrier family 4, sodium bicarbonate transporter-like, member 11 ( MGI:2138987)
Sequence:	sense strand is shown >T37038 GATGTGCAGAAGACCATAGCTGGACAGAGCATCGGAGGCCTCTTGTATGCACTGTTCTCTGGGCAGCCCC TGGTGATACTGTTGACAACCGCACCCCTGGCCATCTACACCCAGGTGATTCGAGTCATCTGTGATGACTA CAATCTGGACTTCAACGCCTTCTATGCATGGACGGGCCTGTGGAACAGTTTCTTCCTTGCACTTTATGCC TTCCTCAACCTCAGCCTGCTTATGAATCTCTTTAAGAGGTCTACAGAGGAAATCATTGCTCTGTTCATTT CCATCACGTTTGTGCTGGATGCTGTCAAGGGCATGGTCAAAATCTTCGGGAAGTACTACTATGGTCACCA CTACCACACAAAAAGGACTTCCTCCTTGGTGAGCCTGTTGGGCATTGGCAGAAGCCCCAACAGCAGTCTC CACACTGCACTCAACGCCAGCCTCTTGGCCAGCCCCGTAGAGATGGCCACAACCAGCAGCCCCGGCAGCA CACACTCGGGCCAGGCTACAGCGGTGCTCAGCCTCCTCATCATGTTGGGCACACTCTGGCTAGGCTACAC CCTCTACCAGTTCAAGAAAAGCCCCTACCTGCACCCATGTGTGCGTGAGACCCTGTCTGACTGTGCCCTG CCCATCGCCGTGCTCTCCTTCTCCCTCATC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 76842. Forward Primer - name:076842_F_cDNA_Slc4a11, sequence:GATGTGCAGAAGACCATAGCTG; Reverse Primer - name:076842_N_SP6_cDNA_Slc4a11, sequence:GATGAGGGAGAAGGAGAGCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20040 same embryo
	EMAGE:20037 same embryo
	EMAGE:20041 same embryo
	EMAGE:20039 same embryo
	EMAGE:20038 same embryo
	EurExpress:euxassay_012145 same experiment
	MGI:4828242 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source