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EMAGE:20088

Svopl SV2 related protein homolog (rat)-like ( MGI:2444335)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20088 EMAGE:20088 EMAGE:20088 EMAGE:20088 EMAGE:20088
"Pseudo-wholemount" of euxassay_012264. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012264_01 euxassay_012264_02 euxassay_012264_03 euxassay_012264_04
EMAGE:20088 EMAGE:20088 EMAGE:20088 EMAGE:20088 EMAGE:20088
euxassay_012264_05 euxassay_012264_06 euxassay_012264_07 euxassay_012264_08 euxassay_012264_09
EMAGE:20088 EMAGE:20088 EMAGE:20088 EMAGE:20088 EMAGE:20088
euxassay_012264_10 euxassay_012264_11 euxassay_012264_12 euxassay_012264_13 euxassay_012264_14
EMAGE:20088 EMAGE:20088 EMAGE:20088 EMAGE:20088 EMAGE:20088
euxassay_012264_15 euxassay_012264_16 euxassay_012264_17 euxassay_012264_18 euxassay_012264_19
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euxassay_012264_20 euxassay_012264_21 euxassay_012264_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20088Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20088_wholemount_strong.wlz
20088_wholemount_moderate.wlz
20088_wholemount_weak.wlz
20088_wholemount_possible.wlz
20088_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20088_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 06 07 08 09 12 13
vomeronasal organ
strong strong
regionalstrong expression: see section 09 weak expression: see section 12
renal cortex
weak weak
regionalweak expression: see section 05 06 07 08 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30414
Entity Detected:Svopl, SV2 related protein homolog (rat)-like ( MGI:2444335)
Sequence:sense strand is shown

>T30414
CACAGGGACCTGGAATCGGAAAGTTCCAAGGAACACAGAGGGGAGCTGTGTGGGGAATCAGTGACCTAGA
TGGCAGCCAAGCAGACAGAGCCCGTGACAATCATCAGCCTTCGGAAACTGAGCCAGGCAGCCCCAGAACC
GCAGCAGAAAGAGACCAAGACATTCACGGTGGAGGATGCGGTGGAGACCATCGGCTTCGGGCGCTTCCAC
ATTGCACTCTTCCTAATCATGGGCAGCACCGGGGTTGTGGAGGCCATGGAGATCATGTTAATAGCAGTTG
TGTCTCCTGTTATCCGATGTGAATGGCAGCTGGAGAACTGGCAGGTGGCATTTGTCACCACGATGGTGTT
TTTTGGCTACATGGTATCCAGCATCCTTTTTGGTCTCCTGGCTGACAGATATGGCCGCTGGAAGATTTTG
CTGCTCTCGTTCCTGTGGGGAGCCTACTTCTCCTTACTGACCTCTTTCTCCCCCTCCTACATCTGGTTTG
TCTTTCTGCGGACGATGGTAGGCTGTGGAGTGTCTGGCCATGCTCAAGGGTTAATCATAAAGACTGAATT
TTTGCCCACAAAATACCGAGGCTACATGCTGCCCTTGTCGCAGGTTTTCTGGCTTGCTGGCTCCCTGCTC
ATCATCAGCATGGCCTCTGTGGTCATCCCCACCATTGGGTGGCGTTGGCTCATCCGCATCGCCTCCATCC
CTGGCATCATCCTCATCATGGCCTTCAAGTTTATCCCTGAATCTGCTCGCTTCAACGTCTCCACTGGGAA
CACCCAGGCTGCACTGAACACCCTAGAGAGCATTGCCAAGATGAACCGCTCAGTCATGCCAGAGGGACAG
CT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6309979), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59434. Forward Primer - name:059434_F_IRAV104_a10_9430071P14Rik, sequence:CACAGGGACCTGGAATCG; Reverse Primer - name:059434_R_SP6_IRAV104_a10_9430071P14Rik, sequence:CAGCTGTCCCTCTGGCAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20091 same embryo
 EMAGE:20090 same embryo
 EMAGE:20087 same embryo
 EMAGE:20089 same embryo
 EurExpress:euxassay_012264 same experiment
 MGI:4828550 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS