EMAGE mouse gene expression spatial database

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EMAGE:20104

Rgs7bp regulator of G-protein signalling 7 binding protein ( MGI:106334)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012208. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012208_01	euxassay_012208_02	euxassay_012208_03	euxassay_012208_04

euxassay_012208_05	euxassay_012208_06	euxassay_012208_07	euxassay_012208_08	euxassay_012208_09

euxassay_012208_10	euxassay_012208_11	euxassay_012208_12	euxassay_012208_13	euxassay_012208_14

euxassay_012208_15	euxassay_012208_16	euxassay_012208_17	euxassay_012208_18	euxassay_012208_19

euxassay_012208_20	euxassay_012208_21	euxassay_012208_22	euxassay_012208_23	euxassay_012208_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20104_wholemount_strong.wlz

20104_wholemount_moderate.wlz

20104_wholemount_weak.wlz

20104_wholemount_possible.wlz

20104_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20104_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 07 08 09 17 18 19 20 21 moderate expression: see section 03 04

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37651
Entity Detected:	Rgs7bp, regulator of G-protein signalling 7 binding protein ( MGI:106334)
Sequence:	sense strand is shown >T37651 GTTGCTGTCTCTGCACTCATTTCCATTTAGTCTTCCATTCTGTCAGAATCGCTAAGTATCAACATCAGAA CATCTTAATTTATAATGCAACAGAGTCATATATGAAAAATACATTTTGTAAATACACAGTAATCCTACTA TCTTCAAACAATGCATATGGGTAGCTAACTTTCTTTTAATCCAATGGTGTCTTTTTAAGTTTCTTGGGAA ATGAAGTTATCCGGTTAAGAAATGATGTAGTAGCATAAACAATTACCCTCAGATGTAAAATTCAATATTA TCACATTTTGTTTGAATTGAAACCTGAAGCATGGGTCCACACATCAGCATAGTGTTAAATCTTATAGGGC ATGCTGGAATTAGCTCAGATCATAAAACTATTTAACATTTTACATTATTAATGAAAAAAGATGTTTTAGT TAAGCTTAGCTTGTCTCATTTTAGATGACTATGCAGATATGACTTTTCCAATGTGTATTCTGTGTCCTGT CTTATGCTTGGACTCTCGAGAACGGGACACATTAAGCAATACTGTATTTTAGAAAAGAGGGAGCCACACG CTGTGTTGTTCTGCTCATGGCTCTGCTGCGCTCTTTTCCCACGAACTTGTAATATGCTACAAATCCTGTT TTATTTCCTCCTGTCCTTCCTTTGACCCTTCCCCCCACCCCCAATGAAATTTCCCAGGAGTCTTTGCACT TTTGTTCCCTGTTCTGACAGATCTCCGAGTCAGACCAGAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66115. Forward Primer - name:066115_F_cDNA_D13Bwg1146e, sequence:GTTGCTGTCTCTGCACTCATTT; Reverse Primer - name:066115_N_SP6_cDNA_D13Bwg1146e, sequence:CCTCTGGTCTGACTCGGAGAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20103 same embryo
	EMAGE:20105 same embryo
	EMAGE:20102 same embryo
	EurExpress:euxassay_012208 same experiment
	MGI:4827724 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source