Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:20112

Ecsit ECSIT homolog (Drosophila) ( MGI:1349469)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20112 EMAGE:20112 EMAGE:20112 EMAGE:20112 EMAGE:20112
"Pseudo-wholemount" of euxassay_012422. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012422_01 euxassay_012422_02 euxassay_012422_03 euxassay_012422_04
EMAGE:20112 EMAGE:20112 EMAGE:20112 EMAGE:20112 EMAGE:20112
euxassay_012422_05 euxassay_012422_06 euxassay_012422_07 euxassay_012422_08 euxassay_012422_09
EMAGE:20112 EMAGE:20112 EMAGE:20112 EMAGE:20112 EMAGE:20112
euxassay_012422_10 euxassay_012422_11 euxassay_012422_12 euxassay_012422_13 euxassay_012422_14
EMAGE:20112 EMAGE:20112 EMAGE:20112 EMAGE:20112 EMAGE:20112
euxassay_012422_15 euxassay_012422_16 euxassay_012422_17 euxassay_012422_18 euxassay_012422_19
EMAGE:20112 EMAGE:20112
euxassay_012422_20 euxassay_012422_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20112Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20112_wholemount_strong.wlz
20112_wholemount_moderate.wlz
20112_wholemount_weak.wlz
20112_wholemount_possible.wlz
20112_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20112_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31306
Entity Detected:Ecsit, ECSIT homolog (Drosophila) ( MGI:1349469)
Sequence:sense strand is shown

>T31306
CGTGGTTGGTTCCTCGACCCCCGGAGCCCCAGAGGAAGCCCATCAAGGTTCCAGCAATGCATGAAGACTC
GTTCAAGCCATCAGGAAACAGGGAGCGGGACAAGGCAAGCTTCCTGAACGCAGTGCGCAGCTTTGGGGCG
CACAATGTGCGCAAGCGCGGCCACGTGGACTTCATCTACCTAGCACTGCGCAAGATGCCAGAGTTTGGTG
TGGAGCGGGACTTGTCAGTATACAACCTGCTGCTGGATGTTTTCCCCAAGGAGGTCTTCCGGCCCCGCAA
CGTTATCCAGCGCATCTTCGTCCACTACCCACGGCAGCAGGAATGTGGGGTCGCAGTCCTGGAGCAGATG
GAACGACACGGGGTCATGCCCAGCGCAGAGACAGAGTTCCTACTGATTCAGATATTCGGGCGCAAAAGTT
ACCCCATGCTCAAGTTCCTGCGGATGAAGCTGTGGTTCACCCGATTCAAGAATATCAACCCCTACCCAGT
GCCCCGAGATCTTCCCCAGGACCCTTTGGACCTGGCCAAGCTAGGCCTGCGACACATGGAGCCTGACCTC
AGTGCTAAGGTCACTGTCTACCAGATGTCTTTGCCCAGTGACTCGACAGGCATGGAAGATCCCACACAGC
CTCACATTGTAGGAATCCAGAGTCCAGATCAGCAAGCTGCCCTGGCCCGCCACAACCCATCCAGGCCTGT
TTTTGTTGAGGGCCCCTTCCCTCTCTGGCTTCGTAATAAGTGTGTCTACTATCACATCCTAAGAGCTGAC
CTGCCACCTCCTGAGGAAGAGAAAGTAGAGGAGATCCCAGAAGAATGGGAGCTGTACTACCCACAGAAGC
TGGACCTGGAATATTCAAGGAGTGGTTGGGAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4924678), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 38102. Forward Primer - name:038102_F_IRAV51-54_E04_Sitpec, sequence:CGTGGTTGGTTCCTCGAC; Reverse Primer - name:038102_R_SP6_IRAV51-54_E04_Sitpec, sequence:CGTCCCAACCACTCCTTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20113 same embryo
 EMAGE:20117 same embryo
 EMAGE:20116 same embryo
 EMAGE:20115 same embryo
 EMAGE:20114 same embryo
 EurExpress:euxassay_012422 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS