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EMAGE:20113

Cyp2c70 cytochrome P450, family 2, subfamily c, polypeptide 70 ( MGI:2385878)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20113 EMAGE:20113 EMAGE:20113 EMAGE:20113 EMAGE:20113
"Pseudo-wholemount" of euxassay_012373. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012373_01 euxassay_012373_02 euxassay_012373_03 euxassay_012373_04
EMAGE:20113 EMAGE:20113 EMAGE:20113 EMAGE:20113 EMAGE:20113
euxassay_012373_05 euxassay_012373_06 euxassay_012373_07 euxassay_012373_08 euxassay_012373_09
EMAGE:20113 EMAGE:20113 EMAGE:20113 EMAGE:20113 EMAGE:20113
euxassay_012373_10 euxassay_012373_11 euxassay_012373_12 euxassay_012373_13 euxassay_012373_14
EMAGE:20113 EMAGE:20113 EMAGE:20113 EMAGE:20113 EMAGE:20113
euxassay_012373_15 euxassay_012373_16 euxassay_012373_17 euxassay_012373_18 euxassay_012373_19
EMAGE:20113 EMAGE:20113 EMAGE:20113 EMAGE:20113
euxassay_012373_20 euxassay_012373_21 euxassay_012373_22 euxassay_012373_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20113Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20113_wholemount_strong.wlz
20113_wholemount_moderate.wlz
20113_wholemount_weak.wlz
20113_wholemount_possible.wlz
20113_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20113_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
heart ventricle
strong strong
spottedstrong expression: see section 06 07 08 09 10 11 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31276
Entity Detected:Cyp2c70, cytochrome P450, family 2, subfamily c, polypeptide 70 ( MGI:2385878)
Sequence:sense strand is shown

>T31276
GGCCCCACTCCTTTACCAATTGTTGGAAATATTTTGCAAGTATATGTTAAAAATATTAGCAAATCTATGG
GCATGCTAGCAAAAAAGTATGGCCCTGTGTTTACTGTATACCTGGGCATGAAGCCCACTGTGGTGCTGCA
TGGATACAAAGCAATGAAGGAAGCTCTGATTGACCAGGGAGATGAGTTTTCTGATAAAACGGATTCATCG
TTACTCAGTAGAACCAGCCAAGGCTTAGGCATCGTCTTCAGCAATGGAGAAACATGGAAACAAACCCGGC
GTTTCTCCCTCATGGTCTTAAGGTCTATGGGGATGGGAAAGAAAACTATTGAAGACCGAATTCAGGAGGA
AATCCTTTATATGTTGGATGCACTGAGAAAAACCAATGGCTCCCCCTGTGATCCTAGCTTCCTTCTGGCC
TGTGTTCCCTGCAATGTGATCTCTACTGTCATTTTCCAGCATCGATTTGACTACAATGATCAGACATTCC
AAGACTTCATGGAGAATTTTCATAGAAAAATAGAAATTTTAGCATCTCCTTGGTCCCAGCTGTGCAGTGC
CTACCCTATTTTATACTATCTCCCTGGAATCCATAACAGATTCTTAAAAGATGTGACTCAACAGAAAAAG
TTCATCTTGGAGGAAATAAACAGGCATCAAAAATCTCTGGACCTCAGTAACCCTCAGGACTTTATTGACT
ACTTCTTGATTAAAATGGAAAAGGAAAAACACAATCAAAAATCTGAATTTACTATGGATAACCTGGTTGT
CAGCATAGGTGATCTTTTTGGAGCCGGAACAGAGACAACGAGTTCCACAGTGAAATATGGGCTTTTGCTC
CTGCTGAAGTACCCAGAAGTCACAGCTAAAATTCAGGAAGAAATTGCTCATGTGATTGGTAGACATCGGA
GACCCACCATGCAGGACAGGAACCACATGCCG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4210859), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 55650. Forward Primer - name:055650_F_IRAV49_h05_Cyp2c70, sequence:GGCCCCACTCCTTTACCA; Reverse Primer - name:055650_R_SP6_IRAV49_h05_Cyp2c70, sequence:ACGGCATGTGGTTCCTGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20117 same embryo
 EMAGE:20112 same embryo
 EMAGE:20116 same embryo
 EMAGE:20115 same embryo
 EMAGE:20114 same embryo
 EurExpress:euxassay_012373 same experiment
 MGI:4824165 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS