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EMAGE:20117

Lcat lecithin cholesterol acyltransferase ( MGI:96755)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20117 EMAGE:20117 EMAGE:20117 EMAGE:20117 EMAGE:20117
"Pseudo-wholemount" of euxassay_012382. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012382_01 euxassay_012382_02 euxassay_012382_03 euxassay_012382_04
EMAGE:20117 EMAGE:20117 EMAGE:20117 EMAGE:20117 EMAGE:20117
euxassay_012382_05 euxassay_012382_06 euxassay_012382_07 euxassay_012382_08 euxassay_012382_09
EMAGE:20117 EMAGE:20117 EMAGE:20117 EMAGE:20117 EMAGE:20117
euxassay_012382_10 euxassay_012382_11 euxassay_012382_12 euxassay_012382_13 euxassay_012382_14
EMAGE:20117 EMAGE:20117 EMAGE:20117 EMAGE:20117 EMAGE:20117
euxassay_012382_15 euxassay_012382_16 euxassay_012382_17 euxassay_012382_18 euxassay_012382_19
EMAGE:20117 EMAGE:20117 EMAGE:20117 EMAGE:20117
euxassay_012382_20 euxassay_012382_21 euxassay_012382_22 euxassay_012382_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20117Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20117_wholemount_strong.wlz
20117_wholemount_moderate.wlz
20117_wholemount_weak.wlz
20117_wholemount_possible.wlz
20117_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20117_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
heart ventricle
strong strong
spottedstrong expression: see section 06 07 08 09 10 11 12 13 14 15
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31277
Entity Detected:Lcat, lecithin cholesterol acyltransferase ( MGI:96755)
Sequence:sense strand is shown

>T31277
GGGTGGACTGCTGGATTGATAATACCAGGATTGTCTACAACCACAGCTCTGGGCGCGTGTCCAATGCCCC
TGGTGTCCAGATCCGTGTCCCTGGCTTTGGCAAGACCGAATCTGTTGAGTACGTGGATGACAACAAGCTA
GCAGGCTACCTGCACACACTGGTGCAGAATCTGGTTAACAACGGATATGTGCGGGATGAGACAGTGCGGG
CCGCACCCTATGACTGGCGTCTGGCACCCCACCAGCAGGATGAATACTACAAGAAGCTGGCTGGCCTGGT
AGAGGAGATGTATGCCGCTTATGGGAAGCCTGTCTTCCTCATTGGGCATAGCCTTGGCTGTCTGCATGTG
CTCCACTTCTTACTGCGGCAGCCTCAGTCCTGGAAGGACCACTTCATTGATGGTTTTATCTCTCTCGGGG
CTCCGTGGGGTGGTTCCATCAAGGCCATGCGGATCCTGGCCTCAGGTGACAACCAGGGCATCCCCATCCT
GTCCAACATAAAGCTGAAAGAGGAGCAGCGCATAACCACGACTTCCCCCTGGATGCTCCCAGCCCCTCAC
GTGTGGCCTGAAGACCATGTGTTCATTTCCACACCAAACTTCAACTACACCGTCCAAGACTTTGAGCGTT
TTTTCACAGATCTGCATTTTGAAGAAGGCTGGCACATGTTTCTTCAGTCTCGTGACCTACTGGAGCGCCT
CCCCGCACCTGGTGTAGAAGTATATTGTCTCTACGGTGTGGGCAGACCCACACCCCACACCTACATCTAT
GACCACAACTTCCCCTACAAAGACCCCGTGGCTGCACTCTATGAAGATGGGGACGACACCGTAGCCACCC
GCAGCACTGAGCTCTGTGGCCAGTGGCAGGGCCGCCAGTCGCAGCCCGTACATTTGCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4212194), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 54938. Forward Primer - name:054938_F_IRAV49_h07_Lcat, sequence:GGGTGGACTGCTGGATTG; Reverse Primer - name:054938_R_SP6_IRAV49_h07_Lcat, sequence:GCAGCAAATGTACGGGCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20113 same embryo
 EMAGE:20112 same embryo
 EMAGE:20116 same embryo
 EMAGE:20115 same embryo
 EMAGE:20114 same embryo
 EurExpress:euxassay_012382 same experiment
 MGI:4825883 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS