EMAGE mouse gene expression spatial database

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EMAGE:20193

Ctbp2 C-terminal binding protein 2 ( MGI:1201686)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012486. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012486_01	euxassay_012486_02	euxassay_012486_03	euxassay_012486_04

euxassay_012486_05	euxassay_012486_06	euxassay_012486_07	euxassay_012486_08	euxassay_012486_09

euxassay_012486_10	euxassay_012486_11	euxassay_012486_12	euxassay_012486_13	euxassay_012486_14

euxassay_012486_15	euxassay_012486_16	euxassay_012486_17	euxassay_012486_18	euxassay_012486_19

euxassay_012486_20	euxassay_012486_21	euxassay_012486_22	euxassay_012486_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20193_wholemount_strong.wlz

20193_wholemount_moderate.wlz

20193_wholemount_weak.wlz

20193_wholemount_possible.wlz

20193_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20193_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20

pancreas

moderate

regional

moderate expression: see section 09 10 11 14 15

vibrissa

weak

regional

weak expression: see section 05 06 07 08 09 20 21 22 23

olfactory cortex marginal layer

weak

homogeneous

weak expression: see section 11 12 13 15 16 17 18

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 12 16 17 18

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23

eyelid

moderate

regional

moderate expression: see section 01 02 03 04 23

midgut

weak

regional

weak expression: see section 12 13 14 15 16 17 18 19 20 21 22 23

lower jaw incisor

moderate

regional

moderate expression: see section 12 14 15 16

lower jaw molar

moderate

regional

moderate expression: see section 08 18 19

upper jaw incisor

moderate

regional

moderate expression: see section 11 15 16

upper jaw molar

moderate

regional

moderate expression: see section 08 09 18 19

metanephros

moderate

regional

moderate expression: see section 05 06 07 08 09 10 14 15 16 17 18 19

lung

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31365
Entity Detected:	Ctbp2, C-terminal binding protein 2 ( MGI:1201686)
Sequence:	sense strand is shown >T31365 CAGCAAGCAATTCACCCCGAGCTCAATGGTGCCACATACAGGTATCCACCCGGCATTGTAGGTGTGGCTC CAGGAGGGCTTCCTCCAGCGATGGAAGGGATCATCCCTGGGGGCATCCCCGTGACTCACAACCTCCCCAC AGTGGCACACCCCTCCCAAGCTCCTTCCCCCAACCAGCCCACAAAACACGGGGACAATCGAGAGCACCCC AACGAGCAATAGCAGAGAATGCCTGAAGCTAATCCGATAAACTTGGGACCAAGAGACAGTAGAAAACGGA TGAATTTGATGGTCTTTTAAACTGATCCTGGACATCACTGATACTGCAGTGTTAAAGCTACAAAAGCTAG AAAACTGAAGATGGTATCTGCTTCCAGAGGTGTGGGAAGACTAGGACGTGATTATTAACGACCAACTTCT GTTATTGTGTGTTAAGTTTTTCATCTGTGCATCAAATCACAAAGAATAAATAGAGCTTTTCCTTTATCAA TCCCCTGGGCACAGCAGGTCCTGCAGAGCTTGCTCTAGAATGTTGCATCAAGAGCTCCGAATATCAATAA AAAGTTGAGAGGAAATCCCCATCAAGACTAGTCCCTTTGGTCTCCAGGGGCTGGTGCCTCTGCTGATAGG AAGATTAAAATTAAGCCAAGATGGGGAGGACATTGTTTGCCTGTGTAGACATCTCCACGCACAGGATTGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4511826), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 36440. Forward Primer - name:036440_F_IRAV55-58_C22_Ctbp2, sequence:CAGCAAGCAATTCACCCC; Reverse Primer - name:036440_R_SP6_IRAV55-58_C22_Ctbp2, sequence:TTCAATCCTGTGCGTGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20196 same embryo
	EMAGE:20195 same embryo
	EMAGE:20194 same embryo
	EMAGE:20192 same embryo
	EMAGE:20191 same embryo
	EurExpress:euxassay_012486 same experiment
	MGI:4824097 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source