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EMAGE:20207

D6Ertd474e DNA segment, Chr 6, ERATO Doi 474, expressed ( MGI:1196315)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20207 EMAGE:20207 EMAGE:20207 EMAGE:20207 EMAGE:20207
"Pseudo-wholemount" of euxassay_012222. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012222_01 euxassay_012222_02 euxassay_012222_03 euxassay_012222_04
EMAGE:20207 EMAGE:20207 EMAGE:20207 EMAGE:20207 EMAGE:20207
euxassay_012222_05 euxassay_012222_06 euxassay_012222_07 euxassay_012222_08 euxassay_012222_09
EMAGE:20207 EMAGE:20207 EMAGE:20207 EMAGE:20207 EMAGE:20207
euxassay_012222_10 euxassay_012222_11 euxassay_012222_12 euxassay_012222_13 euxassay_012222_14
EMAGE:20207 EMAGE:20207 EMAGE:20207 EMAGE:20207 EMAGE:20207
euxassay_012222_15 euxassay_012222_16 euxassay_012222_17 euxassay_012222_18 euxassay_012222_19
EMAGE:20207
euxassay_012222_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20207Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20207_wholemount_strong.wlz
20207_wholemount_moderate.wlz
20207_wholemount_weak.wlz
20207_wholemount_possible.wlz
20207_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20207_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
heart ventricle
strong strong
spottedstrong expression: see section 09 12 16 moderate expression: see section 10 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37660
Entity Detected:D6Ertd474e, DNA segment, Chr 6, ERATO Doi 474, expressed ( MGI:1196315)
Sequence:sense strand is shown

>T37660
CCTGTGTTGCAAGACTCAAAAGAGGGAGAGAGAGAGTTGGCTCAGCTGGTGAAGAGCTTGATGGACATGA
AGACCTGAGCTTGGATCTCCCAACATCTTTGTGAAAGGACGGGCACAGCCCCTGAATCTCCAGAGCTGTG
ACAATGGAAGCAATGGCTCCTCAGAGCTTGCTGGCCAGCCAACCTGCTTGAGTTCAGCTCTAGATTCTCT
GTGACCCTGACTCAAAAACTAAGGCGTGGAGTGACTGAAGAAGAGATCTGATGTCAACCACTTGTTCTTA
TTTACACACACACACACACACACACACACACACACACACACTCTTAAATAAAAATTAAATTATTTGTTGA
AAATGACAAAAAGATGGACACCATACAGTTAGAAGTGGCAGCTACCAAGGGACTTGTCTTGTGGTGCTCT
TTTATCAACAGTTTCTGATTCCTTTGACATAGCAAGTGGCAGTCCTGGGAGCCGGTTTCCAGTCACAAAT
CCCAGTTCCCTTTCTCCCCGTTTTGTTCAGGTTCTGCCACTGTCTTGAGTATGCTCTTTTGTGCTTACTA
AAGCTGTGCTGTGTGGGAGAGTCATCTGTTTGTTCCTTGTTCTTCAGTGGGCTGAGTGATGGACCCCTCC
CTCTTTACAGATAATAGATCTGTGAGAAATCTCACACCACTGGGACCTTATTTGGGCTTAGAGATTTGCT
GGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 80043. Forward Primer - name:080043_F_cDNA_D6Ertd474e, sequence:CCTGTGTTGCAAGACTCAAAAG; Reverse Primer - name:080043_N_SP6_cDNA_D6Ertd474e, sequence:ACCAGCAAATCTCTAAGCCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20206 same embryo
 EMAGE:20204 same embryo
 EMAGE:20202 same embryo
 EMAGE:20203 same embryo
 EMAGE:20205 same embryo
 EurExpress:euxassay_012222 same experiment
 MGI:4824208 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS