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EMAGE:20210

Zc3hc1 zinc finger, C3HC type 1 ( MGI:1916023)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20210 EMAGE:20210 EMAGE:20210 EMAGE:20210 EMAGE:20210
"Pseudo-wholemount" of euxassay_012316. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012316_01 euxassay_012316_02 euxassay_012316_03 euxassay_012316_04
EMAGE:20210 EMAGE:20210 EMAGE:20210 EMAGE:20210 EMAGE:20210
euxassay_012316_05 euxassay_012316_06 euxassay_012316_07 euxassay_012316_08 euxassay_012316_09
EMAGE:20210 EMAGE:20210 EMAGE:20210 EMAGE:20210 EMAGE:20210
euxassay_012316_10 euxassay_012316_11 euxassay_012316_12 euxassay_012316_13 euxassay_012316_14
EMAGE:20210 EMAGE:20210 EMAGE:20210 EMAGE:20210 EMAGE:20210
euxassay_012316_15 euxassay_012316_16 euxassay_012316_17 euxassay_012316_18 euxassay_012316_19
EMAGE:20210 EMAGE:20210
euxassay_012316_20 euxassay_012316_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20210Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20210_wholemount_strong.wlz
20210_wholemount_moderate.wlz
20210_wholemount_weak.wlz
20210_wholemount_possible.wlz
20210_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20210_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30465
Entity Detected:Zc3hc1, zinc finger, C3HC type 1 ( MGI:1916023)
Sequence:sense strand is shown

>T30465
GGATCGCCTCAAGCAGAACAGTCTCCATTGGAATCCACAAGCAAAGAAGCCTTCTTTCACAGAGTGGAAA
CCTTTTCTTCTTTGAAGTGGGCAGGGAAGCCCCCTGAGCTGTCTCCACTTATCTGCGCTAAATATGGTTG
GGTCACAGTTGAATGTGACATGCTCAAGTGCTCCAGCTGCCAGGCTTTCCTCTGTGCCAGCCTGCAGCCC
ACGTTTGACTTTGGCAGATATAAGGAGCGATGTGCTGAGCTCAAGAAATCCTTGTGCAGTGCCCATGAGA
AGTTCTGTTTTTGGCCAGACAGCCCATCTCCAGATAGATTTGGGATGTTGCCATTGGGTGAGCCTGCTGT
TCTCATCAGTGAGTTCCTGGACCGGTTTCAAAGCCTTTGCCATTTGGACCTCCAACTTCCTTCTCTGAGG
CCAGAGGACTTGAAAACTATGTGCTTGACAGAAGACGCGGTCAGTGCCCTGCTGCACCTGCTGGAGGATG
AGCTTGATTTCCACGCCGATGACAGGAAAACTACAAGCAAGTTAGGCTCCGACGTCCAAGTCCAAGCCAC
TGCGTGTGTTCTCTCTCTGTGCGGCTGGGCGTGTAGTTCCTTGGAACCTACTCAGCTCTCCCTGATAACA
TGTTACCAGTGTATGAGGAAGGTCGGTCTCTGGGGCTTTCAGCAGATTGAATCATCCATGACAGATCTGG
AGGCTTCCTTTGGCCTGACCAGCTCCCCGATTCCGGGTGTGGAGGGCCGGCCTGAACACTTCCCTCTGGT
GCCTGAATCCCCTAGGCGGATGATGACCCGAAGCCAGGATGCTACAGTTTCTCCAGGCTCTGAGCAGTCT
GAAAAAAGTCCTGGTCCTATTGTTTCCCGAACCCGGAGTTGGGAGTCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30011212), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58873. Forward Primer - name:058873_F_IRAV106_g11_Nipa, sequence:GGATCGCCTCAAGCAGAA; Reverse Primer - name:058873_R_SP6_IRAV106_g11_Nipa, sequence:AAGACTCCCAACTCCGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20208 same embryo
 EMAGE:20212 same embryo
 EMAGE:20211 same embryo
 EMAGE:20209 same embryo
 EurExpress:euxassay_012316 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS