EMAGE mouse gene expression spatial database

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EMAGE:20213

Mtmr10 myotubularin related protein 10 ( MGI:2142292)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012297. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012297_01	euxassay_012297_02	euxassay_012297_03	euxassay_012297_04

euxassay_012297_05	euxassay_012297_06	euxassay_012297_07	euxassay_012297_08	euxassay_012297_09

euxassay_012297_10	euxassay_012297_11	euxassay_012297_12	euxassay_012297_13	euxassay_012297_14

euxassay_012297_15	euxassay_012297_16	euxassay_012297_17	euxassay_012297_18	euxassay_012297_19

euxassay_012297_20	euxassay_012297_21	euxassay_012297_22	euxassay_012297_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20213_wholemount_strong.wlz

20213_wholemount_moderate.wlz

20213_wholemount_weak.wlz

20213_wholemount_possible.wlz

20213_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20213_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 04 05 06 07 08 21 22 23

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 10 11 15 16

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14 weak expression: see section 09 10

pons ventricular layer

moderate

homogeneous

moderate expression: see section 11 weak expression: see section 06 07 08 09 10 12 13 14 15 16 17 18

midbrain ventricular layer

moderate

regional

moderate expression: see section 11 weak expression: see section 05 06 07 08 09 10 12 13 14 15 16 17

spinal cord ventricular layer

moderate

regional

moderate expression: see section 13 weak expression: see section 14 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17 weak expression: see section 08 18

vomeronasal organ

moderate

regional

moderate expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30473
Entity Detected:	Mtmr10, myotubularin related protein 10 ( MGI:2142292)
Sequence:	sense strand is shown >T30473 TCTCCAGACCAGCCAACCTACACGGCATTATCCTGCCACGTCTCTCCGGAACACACATAAAATTGTGGAA ACTGTGCTACTTCCGTTGGGTCCCTGAGGCCCAGATCAACCTCGGGGGCTCCATCATGGCCTTCCACAAG CTCTCTCTCCTGGCTGATGAGGTGGACATGCTGAGCAGGATGCTGAGGCAGCACCGCAGCGGCCCCTTGG AAGCCTGCTATGCAGAGCTGGACCAGAGCAGGATGTACTTCCGGGCCACGGGGCCACATGACACGCTGGG GACACCAGAGTTCCTCTCCTCTTCATTTCCATTTTCTCCTGTAGGAAATCTGTGTAGACGAAGCATTTTA GGAACGCCATTAAGCAAATTTTTAAGTGGGGCCAAAATATGGTTATCTACTGAGACACTAGCAAATGAAG ACTAAATTAAGGTATTTTTCTGGACATATTGAGAGAAGCTGGACATTTCCCTCTCTGAACTGAAATTGCT AACTGAGGCATTTAAAACATTTTATATGTAAGAAATTTGCACAAATATTTAAACCATGTTTAATCATGCT GCCTTCAGTTATTCTAAGGGCAGTGTGACTTTTGGGTTTGACCTTTGGGTCTGGCTCATTTGGGTCATTT GCAGCACGAATTTCCCATTGTTACACTTGATATCAAAATATTGAGGTTCCTCTGCCATGCGAATTTTGCC AACTTCTATTTTCCAACCTACTAAAGGTGATCTGATAGTCAGATTACAACCCAGAGAAAGTGGATAGGGA AGTTAATAAATTAGTGTATTTAATGCATTTCTAGATCATCTGATAAAACTCCCAAGAGGCCTCAGTTGGC ATCCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30052208), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58884. Forward Primer - name:058884_F_IRAV107_d04_BB128963, sequence:TCTCCAGACCAGCCAACC; Reverse Primer - name:058884_R_SP6_IRAV107_d04_BB128963, sequence:GTGGATGCCAACTGAGGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20214 same embryo
	EMAGE:20216 same embryo
	EMAGE:20215 same embryo
	EMAGE:20217 same embryo
	EurExpress:euxassay_012297 same experiment
	MGI:4826495 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source