EMAGE mouse gene expression spatial database

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EMAGE:20219

Pax3 paired box gene 3 ( MGI:97487)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012277. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012277_01	euxassay_012277_02	euxassay_012277_03	euxassay_012277_04

euxassay_012277_05	euxassay_012277_06	euxassay_012277_07	euxassay_012277_08	euxassay_012277_09

euxassay_012277_10	euxassay_012277_11	euxassay_012277_12	euxassay_012277_13	euxassay_012277_14

euxassay_012277_15	euxassay_012277_16	euxassay_012277_17	euxassay_012277_18	euxassay_012277_19

euxassay_012277_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20219_wholemount_strong.wlz

20219_wholemount_moderate.wlz

20219_wholemount_weak.wlz

20219_wholemount_possible.wlz

20219_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20219_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 01 02 14 15 16 17

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 12 13

diencephalon roof plate

strong

regional

strong expression: see section 11

telencephalon mantle layer

moderate

single cell

moderate expression: see section 05 weak expression: see section 04

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 13 14 weak expression: see section 12

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 04 05 07 08 11 15 16 17 moderate expression: see section 06 09 10 12 13 14 18

pons ventricular layer

strong

regional

strong expression: see section 07 15 16 moderate expression: see section 06 14

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 weak expression: see section 12

nose

moderate

regional

moderate expression: see section 06 07 11 weak expression: see section 04

naris

moderate

regional

moderate expression: see section 09 10

viscerocranium

moderate

regional

Expression in the turbinate bone.

lower lip

moderate

regional

moderate expression: see section 04 05 06 07 10 11 12 13 weak expression: see section 09 14

upper lip

moderate

regional

moderate expression: see section 03 04 05 10 11 12 13 weak expression: see section 09 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30426
Entity Detected:	Pax3, paired box gene 3 ( MGI:97487)
Sequence:	sense strand is shown >T30426 CAACCATCTCATTCCGGGGGGATTCCCTCCCACCGCCATGCCGACCCTGCCAACATACCAGCTGTCGGAG ACCTCTTACCAGCCCACGTCTATTCCACAAGCCGTGTCAGATCCCAGTAGCACCGTCCACAGACCTCAGC CGCTTCCTCCGAGCACTGTACACCAAAGCACTATTCCTTCGAACGCAGACAGCAGCTCTGCCTACTGCCT CCCCAGCACCAGGCATGGATTTTCAAGCTATACAGACAGCTTTGTGCCTCCATCGGGGCCCTCCAACCCC ATGAACCCCACCATCGGCAATGGCCTTTCACCTCAGGTAATGGGACTTCTGACCAACCACGGTGGGGTAC CGCACCAGCCTCAGACCGACTATGCTCTCTCCCCTCTCACTGGGGGCCTGGAACCCACGACCACGGTGTC AGCCAGCTGCAGTCAGAGACTGGAACATATGAAGAATGTGGACAGTCTGCCCACATCTCAGCCCTATTGT CCCCCCACCTATAGCACCGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6518115), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58562. Forward Primer - name:058562_F_IRAV105_a04_Pax3, sequence:CAACCATCTCATTCCGGG; Reverse Primer - name:058562_R_SP6_IRAV105_a04_Pax3, sequence:TGCGGTGCTATAGGTGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20220 same embryo
	EMAGE:20218 same embryo
	EurExpress:euxassay_012277 same experiment
	MGI:4827063 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source