EMAGE mouse gene expression spatial database

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EMAGE:20227

Ap3b2 adaptor-related protein complex 3, beta 2 subunit ( MGI:1100869)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001991. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001991_01	euxassay_001991_02	euxassay_001991_03	euxassay_001991_04

euxassay_001991_05	euxassay_001991_06	euxassay_001991_07	euxassay_001991_08	euxassay_001991_09

euxassay_001991_10	euxassay_001991_11	euxassay_001991_12	euxassay_001991_13	euxassay_001991_14

euxassay_001991_15	euxassay_001991_16	euxassay_001991_17	euxassay_001991_18	euxassay_001991_19

euxassay_001991_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20227_wholemount_strong.wlz

20227_wholemount_moderate.wlz

20227_wholemount_weak.wlz

20227_wholemount_possible.wlz

20227_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20227_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 06 07 08 14 15 not examined expression: see section 13

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 15 16 17

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 03 04 06 14 15

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 14 15 16 17 18

vagus x ganglion

strong

regional

strong expression: see section 05 12 13

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 02 03 04 05 06 14 15 16

trigeminal v nerve

strong

regional

strong expression: see section 07 08 14

spinal cord

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

strong

regional

strong expression: see section 06 11 12

thoracic ganglion

strong

regional

strong expression: see section 09 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 12 13 14 15

not examined

regional

not examined expression: see section 01 02 03 19 20

neural retina

strong

regional

strong expression: see section 01 02 19 20

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 12 13 14 15 16

vomeronasal organ

strong

regional

strong expression: see section 10 12

testis

moderate

regional

moderate expression: see section 03 weak expression: see section 04 05 17 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2486
Entity Detected:	Ap3b2, adaptor-related protein complex 3, beta 2 subunit ( MGI:1100869)
Sequence:	sense strand is shown >T2486 CTCGAGGCCAGATTCGGCACGAGGGCATGTGGGCACTCCCAAGCTGCCTGCTGGCATCAGCATTCAAGAA TTCCCTGAAATCGAGTCCCTGGCACCTGGAGAATCCACCACTACTGTGATGGGCATTAACTTCTGCGACT CAACCCAGGCAGCCAACTTCCAGCTGTGCACCCAAACCCGACAGTTCTATGTCTCCATTCAGCCACCTGT CGGGGAGCTGATGGCCCCTGTATTTATGAGTGAGAATGAGTTCAAGAAGGAACAGGGAAAGCTGACAGGC ATGAATGAGATCACAGAGAAGCTTACATTGCCAGACACCTGTCGAAGTGATCACATGGTGGTGCAGAAAG TGACTGCCACCGCCAACCTAGGTCGTGTCCCATGTGGGACATCTGATGAGTACAGGTTTGCAGGGAGGAC ATTAACTAGTGGGAGTCTGGTCCTGCTGACTCTGGATGCCCGGGCAGCTGGAGCCGCCCAGCTGACCGTC AACAGTGAGAAGATGGTGATTGGCACCATGCTGGTGAAGGATGTGATTCAGGCTCTGACCCAGTGACTTC TCAGCAC
Notes:	The probe template was PCR amplified from IMAGE:1263332 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1263332 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4823163 same experiment
	EurExpress:euxassay_001991 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source