EMAGE mouse gene expression spatial database

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EMAGE:20235

Trmt6 tRNA methyltransferase 6 homolog (S. cerevisiae) ( MGI:1914176)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012323. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012323_01	euxassay_012323_02	euxassay_012323_03	euxassay_012323_04

euxassay_012323_05	euxassay_012323_06	euxassay_012323_07	euxassay_012323_08	euxassay_012323_09

euxassay_012323_10	euxassay_012323_11	euxassay_012323_12	euxassay_012323_13	euxassay_012323_14

euxassay_012323_15	euxassay_012323_16	euxassay_012323_17	euxassay_012323_18	euxassay_012323_19

euxassay_012323_20	euxassay_012323_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20235_wholemount_strong.wlz

20235_wholemount_moderate.wlz

20235_wholemount_weak.wlz

20235_wholemount_possible.wlz

20235_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20235_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 10 11 12 13

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 14 15 16

telencephalon ventricular layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30479
Entity Detected:	Trmt6, tRNA methyltransferase 6 homolog (S. cerevisiae) ( MGI:1914176)
Sequence:	sense strand is shown >T30479 ACCCACACAAGGCTGAGGAGCCAGCAGCTAAAAAACAGAAATGCATGGAATCTGCCTCTTAACTCTTAGG AGATTAATGTTTGTTCCAAGGCATTCTGCAGCTGGTAATTACACTTTAAGTGTCATCACTAAAATTTTCC CCTTGTCCTAAGAGTGTGTGTACACCTGTTCCTGGGAAGAAGTAAGCCTTTCGTTTGCCTCTGTTGCAGA AGGATTAGTCAGGGCTGCAACTCTGATGGACAGTAATGGATTGGTACAGCAGTCCTGTATCACAGCAGGC TCGGTACTTAAAGGATCTGCAGATTCCTTGGGAATGTCAACACCTTGAAAATGTTGATGTGTAAGTCTTT GTTTATATTTTAAGCAAAGATGATAATACAAACTAGCCACAACATGGTGAGCAGTGAAAGGAAATTTCCT CTCATCCACTCTTCCTCCTAAGCAATATGGATGTGTGTGCCTGTGAGATTTACAGTACCAAAACGTCTAC CCTTGCCCTGCAATGAAAGAAGCTTTTTTTTAGATACTAGATTTTGTTATTTTAATAGTCAATGGCCTGA GGAAGATCCAGGTATTTCTTTTCCTGCCTCTCCTTTTTTGGTACATAGAAAGGGTGGTGGGTGCTCCTTT GCGGTGAAAGGGTCTCCTCAGTTCTGGGTGAAGTGATTGGAACTTCTGTGTACTATTAAACAATTGGGTT TTTCTCTCTCTAGACACAGACACACAGAAACCTGTATCAGTGTTGGTATATATGCATACACATAGATGTA TCTATATGTCAGCATGGACAGAATGTACTACCTGTGGTGGGACAGAGAAGGAATACACGTTTATTTGAAC TGTGGTAAAGACTTGTGCAGGACAGAGCAGCTCTTGGTGTGTTGCTGTTACTCTTGTCAAGTATGTTTCA AGTGCCTTCTGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30030801), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58880. Forward Primer - name:058880_F_IRAV107_f06_3300001M20Rik, sequence:ACCCACACAAGGCTGAGG; Reverse Primer - name:058880_R_SP6_IRAV107_f06_3300001M20Rik, sequence:CCCCAGAAGGCACTTGAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20237 same embryo
	EMAGE:20236 same embryo
	EMAGE:20240 same embryo
	EMAGE:20239 same embryo
	EMAGE:20238 same embryo
	EurExpress:euxassay_012323 same experiment
	MGI:4828930 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source