EMAGE mouse gene expression spatial database

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EMAGE:20270

Il18r1 interleukin 18 receptor 1 ( MGI:105383)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012379. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012379_01	euxassay_012379_02	euxassay_012379_03	euxassay_012379_04

euxassay_012379_05	euxassay_012379_06	euxassay_012379_07	euxassay_012379_08	euxassay_012379_09

euxassay_012379_10	euxassay_012379_11	euxassay_012379_12	euxassay_012379_13	euxassay_012379_14

euxassay_012379_15	euxassay_012379_16	euxassay_012379_17	euxassay_012379_18	euxassay_012379_19

euxassay_012379_20	euxassay_012379_21	euxassay_012379_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20270_wholemount_strong.wlz

20270_wholemount_moderate.wlz

20270_wholemount_weak.wlz

20270_wholemount_possible.wlz

20270_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20270_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

spotted

moderate expression: see section 11 13 14 weak expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31279
Entity Detected:	Il18r1, interleukin 18 receptor 1 ( MGI:105383)
Sequence:	sense strand is shown >T31279 AACAGAAGCTCGCCCAGAGTCACTTTTCATGATCACACCTTGGAATTCTGGCCAGTTGAGATGGAGGATG AGGGAACGTACATTTCTCAAGTCGGAAATGATCGTCGCAATTGGACCTTAAATGTCACCAAAAGAAACAA ACACAGCTGTTTCTCTGACAAGCTCGTGACAAGCAGAGATGTTGAAGTTAACAAATCTCTGCATATCACT TGTAAGAATCCTAACTATGAAGAGCTGATCCAGGACACATGGCTGTATAAGAACTGTAAGGAAATATCCA AAACCCCAAGGATCCTGAAGGATGCCGAGTTTGGAGATGAGGGCTACTACTCCTGCGTGTTTTCTGTCCA CCATAATGGGACACGGTACAACATCACCAAGACTGTCAATATAACAGTTATTGAAGGAAGGAGTAAAGTA ACTCCAGCTATTTTAGGACCAAAGTGTGAGAAGGTTGGTGTAGAACTAGGAAAGGATGTGGAGTTGAACT GCAGTGCTTCATTGAATAAAGACGATCTGTTTTATTGGAGCATCAGGAAAGAGGACAGCTCAGACCCTAA TGTGCAAGAAGACAGGAAGGAGACGACAACATGGATTTCTGAAGGCAAACTGCATGCTTCAAAAATACTG AGATTTCAGAAAATTACTGAAAACTATCTCAATGTTTTATATAATTGCACCGTGGCCAACGAAGAAGCCA TAGACACCAAGAGCTTCGTCTTGGTGAGAAAAGAAATACCTGATATCCCAGGCCATGTCTTTACAGGAGG AGTAACTGTGCTTGTTCTCGCCTCTGTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4483261), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 54263. Forward Primer - name:054263_F_IRAV50_a01_Il18r1, sequence:AACAGAAGCTCGCCCAGA; Reverse Primer - name:054263_R_SP6_IRAV50_a01_Il18r1, sequence:GCCACAGAGGCGAGAACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20272 same embryo
	EMAGE:20275 same embryo
	EMAGE:20273 same embryo
	EMAGE:20271 same embryo
	EMAGE:20274 same embryo
	EurExpress:euxassay_012379 same experiment
	MGI:4825573 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source