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EMAGE:20287

Tgm1 transglutaminase 1, K polypeptide ( MGI:98730)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20287 EMAGE:20287 EMAGE:20287 EMAGE:20287 EMAGE:20287
"Pseudo-wholemount" of euxassay_012561. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012561_01 euxassay_012561_02 euxassay_012561_03 euxassay_012561_04
EMAGE:20287 EMAGE:20287 EMAGE:20287 EMAGE:20287 EMAGE:20287
euxassay_012561_05 euxassay_012561_06 euxassay_012561_07 euxassay_012561_08 euxassay_012561_09
EMAGE:20287 EMAGE:20287 EMAGE:20287 EMAGE:20287 EMAGE:20287
euxassay_012561_10 euxassay_012561_11 euxassay_012561_12 euxassay_012561_13 euxassay_012561_14
EMAGE:20287 EMAGE:20287 EMAGE:20287 EMAGE:20287 EMAGE:20287
euxassay_012561_15 euxassay_012561_16 euxassay_012561_17 euxassay_012561_18 euxassay_012561_19
EMAGE:20287 EMAGE:20287 EMAGE:20287
euxassay_012561_20 euxassay_012561_21 euxassay_012561_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20287Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20287_wholemount_strong.wlz
20287_wholemount_moderate.wlz
20287_wholemount_weak.wlz
20287_wholemount_possible.wlz
20287_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20287_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
external naris epithelium
strong strong
regionalstrong expression: see section 08 09 13 14
stomach
moderate moderate
regionalmoderate expression: see section 03 04 06 07 weak expression: see section 05
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31422
Entity Detected:Tgm1, transglutaminase 1, K polypeptide ( MGI:98730)
Sequence:sense strand is shown

>T31422
CTGCCATGGTGAACTCCCTGGATGACAATGGAGTTCTGATTGGGAACTGGACCGGTGACTACTCTCGAGG
CACCAACCCCTCAGCGTGGGTGGGCAGTGTGGAGATCCTGCTCAGCTACCTACGCACCGGCTATTCCGTC
CCCTACGGCCAATGCTGGGTCTTTGCCGGTGTGACCACCACAGTGCTCCGATGTCTGGGCTTCGCTACCC
GTACCGTCACCAACTTCAACTCTGCACACGACACAGACACCTCCCTCACTATGGACATCTACTTTGATGA
GAACATGAAGCCACTTGAACATCTGAACCATGATTCTGTTTGGAACTTCCACGTGTGGAACGACTGCTGG
ATGAAGAGGCCAGATCTGCCCTCAGGCTTTGATGGGTGGCAGGTTGTGGATGCCACACCCCAGGAGACCA
GCAGTGGCATCTTCTGCTGTGGCCCCTGTTCTGTGGAGTCCGTCAAGAACGGCTTAGTCTACATGAAGTA
TGACACACCTTTCATTTTTGCTGAGGTAAATAGCGATAAGGTATACTGGCAGCGGCAGGATGATGGCAGC
TTTAAGATAGTGTACGTGGAAGAGAAAGCCATTGGCACACTCATTGTCACAAAGGCGATCCACTCCAACA
ATCGAGAGGACATCACCCACATCTATAAGCACCCAGAAGGCTCAGAAGCAGAGCGGAGGGCTGTGGAGAA
GGCGGCAGCCCATGGCAGCAAACCTAATGTGTATGCCACCCGGGACTCCGCTGAGGATGTGGCAATGCAG
GTGGAGGCGCAGGACGCTGTGATGGGGCAGGATCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4221991), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26518. Forward Primer - name:026518_F_IRAV59-62_B15_Tgm1, sequence:CTGCCATGGTGAACTCCC; Reverse Primer - name:026518_R_SP6_IRAV59-62_B15_Tgm1, sequence:CAGATCCTGCCCCATCAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20288 same embryo
 EMAGE:20292 same embryo
 EMAGE:20290 same embryo
 EMAGE:20291 same embryo
 EMAGE:20289 same embryo
 EurExpress:euxassay_012561 same experiment
 MGI:4828684 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS