EMAGE mouse gene expression spatial database

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EMAGE:20338

2010321M09Rik RIKEN cDNA 2010321M09 gene ( MGI:1917132)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012537. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012537_01	euxassay_012537_02	euxassay_012537_03	euxassay_012537_04

euxassay_012537_05	euxassay_012537_06	euxassay_012537_07	euxassay_012537_08	euxassay_012537_09

euxassay_012537_10	euxassay_012537_11	euxassay_012537_12	euxassay_012537_13	euxassay_012537_14

euxassay_012537_15	euxassay_012537_16	euxassay_012537_17	euxassay_012537_18	euxassay_012537_19

euxassay_012537_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20338_wholemount_strong.wlz

20338_wholemount_moderate.wlz

20338_wholemount_weak.wlz

20338_wholemount_possible.wlz

20338_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20338_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart ventricle

strong

spotted

strong expression: see section 06 07 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31333
Entity Detected:	2010321M09Rik, RIKEN cDNA 2010321M09 gene ( MGI:1917132)
Sequence:	sense strand is shown >T31333 GACACAGCCCACCCAGACGCCGCATTCCAGCTCACCCACGCTGCCCAGCAGCTCAAGCTGGCCAGCACTG AGTATGCCATATATGACCACAACATCACACCCCTGCACACAGACTTCTCAGGGAGCAGCACTGAAAGAAT GTGATGCTGAGCTCCGGAGCGTTCCTTCATGTTCACTTTGTCTGAAGGCAATTTAGAGTTAAAACCTTCT CCAGCTCTATCAGAAACATCATTTGCCCGTCTGAATGTTTCTACTGCAGTCTGAGAAAGGTCTGAAGGCT GGTTGTCTGGGGCTCAGTCTCTAGGTCCAGAAACAGCCTTTGTTCCATGATAGGCACAGCTCCTGAGAAG CTTGGACAGATGAGGAGCGAGCGCAGGAAGAAGCCTAGGCAGCCAGGGCCGTCTAGCATCCAGAAAGCGA CTCTTCATGTCTCAATTACAAGGACATTCTTAAACTTCTGTGCTTGGAAGAAGCAATCCAGATGTCTAGA ACTTGCCTAAGTACAAACCCGATGCCATTGAGTTTCAATTTTTTTCTTAGAACTTGTTAAAATACAAGTG GCAATGCTCAGGTGCTTCTGGTGTATCCCAAGGTACACATTTTTAGACTGTAACCACAATATGTGTAAAA TCACTCCTGTTCATGTTTTTAAATGAAAATTAAATATTTGATTTTCTATAGTGATTGCACTTAGAAACAA ATGGTGTTTTGATCTATCTTATCCCTTGAATATAATATAGCTTCCAATGTCAGACTCCTAAGAAGCAATG TCTCTTGGGTCCTGTGTAACTTCCTAGTTTCTGACAGTGATCAAGCTTTGTACCTCAGCAAGGTTCTGGC CAGTGGTTCTGAGAGCACTGATGGGAGAGTCAGGCCTCCCAGGCTGTGCCTGCACAGTGCTCACCCAGTG CTTCGCGACTGCCTTCAGTACTCCCTTAGGCTCCTGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3602181), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 21646. Forward Primer - name:021646_F_IRAV51-54_J05_2010321M09Rik, sequence:GACACAGCCCACCCAGAC; Reverse Primer - name:021646_R_SP6_IRAV51-54_J05_2010321M09Rik, sequence:GGCAGGAGCCTAAGGGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20341 same embryo
	EMAGE:20339 same embryo
	EMAGE:20340 same embryo
	EMAGE:20337 same embryo
	EurExpress:euxassay_012537 same experiment
	MGI:4822648 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source