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EMAGE:20342

Hao2 hydroxyacid oxidase 2 ( MGI:96012)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20342 EMAGE:20342 EMAGE:20342 EMAGE:20342 EMAGE:20342
"Pseudo-wholemount" of euxassay_012526. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012526_01 euxassay_012526_02 euxassay_012526_03 euxassay_012526_04
EMAGE:20342 EMAGE:20342 EMAGE:20342 EMAGE:20342 EMAGE:20342
euxassay_012526_05 euxassay_012526_06 euxassay_012526_07 euxassay_012526_08 euxassay_012526_09
EMAGE:20342 EMAGE:20342 EMAGE:20342 EMAGE:20342 EMAGE:20342
euxassay_012526_10 euxassay_012526_11 euxassay_012526_12 euxassay_012526_13 euxassay_012526_14
EMAGE:20342 EMAGE:20342 EMAGE:20342 EMAGE:20342 EMAGE:20342
euxassay_012526_15 euxassay_012526_16 euxassay_012526_17 euxassay_012526_18 euxassay_012526_19
EMAGE:20342 EMAGE:20342
euxassay_012526_20 euxassay_012526_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20342Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20342_wholemount_strong.wlz
20342_wholemount_moderate.wlz
20342_wholemount_weak.wlz
20342_wholemount_possible.wlz
20342_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20342_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
adrenal medulla
moderate moderate
regionalmoderate expression: see section 08 09 10 16 17 18
metanephros
moderate moderate
regionalmoderate expression: see section 08 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31398
Entity Detected:Hao2, hydroxyacid oxidase 2 ( MGI:96012)
Sequence:sense strand is shown

>T31398
GATCAATGCCCCCATTTGCATCTCACCCACAGCCTTTCATTCAATTGCCTGGGCAGATGGAGAAAAGAGC
ACAGCTAAAGCTGCTCAGAAGGCCAACATCTGCTATGTCATCAGCAGTTATGCCAGCTATACTGTGGAAG
ACATTGTTGCTGCTGCCCCTGGAGGCCTCCATTGGTTCCAACTTTATGTGCAGCCAGACTGGGACATCAA
CAAGCAGATGGTTCAGAGGATAGAAGCCTTGGGTTTCAAAGCTTTGGTGGTCACTGTAGATGCGCCTGTA
CTTGGCAATAGGCGAGGGAACAAGAGAAGCCTGCTGGATTTGGAGGCAAACATAAAGCTGAAGGATCTCC
GATCCCCTGGAGAGAGCAAGTCTGGACTTCCTACTCCCCTGTCTATGCCAAGTACATCTTCCTGCTGGAA
TGATCTCCCCTTGCTTCAGAGTATGACTCGGTTGCCCATTATCCTCAAAGGGATTTTGACAAAAGAGGAT
GCAGAATTAGCAGTGAAGCACAACATCCGAGGCATCATTGTTTCCAACCATGGCGGGAGGCAGCTTGATG
AGGTTCCTGCATCTATTGATGCTCTGAGAGAAGTGGTGGCTGCCGTCAATGGGAAAATTGAAGTGTACAT
GGATGGTGGGGTTCGAACCGGCAATGATGTGTTGAAGGCACTAGCCCTTGGAGCTAGGTGCATTTTTCTT
GGGAGACCAATCATTTGGGGCCTTGCCTGCAAGGGTGAAGATGGTGTTAAGGAAGTTTTAGATATTCTAA
AAGAAGAACTCCACACTTGTATGGCCCTTTCAGGCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5064100), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 36560. Forward Primer - name:036560_F_IRAV55-58_L20_Hao3, sequence:GATCAATGCCCCCATTTG; Reverse Primer - name:036560_R_SP6_IRAV55-58_L20_Hao3, sequence:CAGCCTGAAAGGGCCATA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20344 same embryo
 EMAGE:20343 same embryo
 EurExpress:euxassay_012526 same experiment
 MGI:4825319 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS