EMAGE mouse gene expression spatial database

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EMAGE:20399

Mt3 metallothionein 3 ( MGI:97173)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012667. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012667_01	euxassay_012667_02	euxassay_012667_03	euxassay_012667_04

euxassay_012667_05	euxassay_012667_06	euxassay_012667_07	euxassay_012667_08	euxassay_012667_09

euxassay_012667_10	euxassay_012667_11	euxassay_012667_12	euxassay_012667_13	euxassay_012667_14

euxassay_012667_15	euxassay_012667_16	euxassay_012667_17	euxassay_012667_18	euxassay_012667_19

euxassay_012667_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20399_wholemount_strong.wlz

20399_wholemount_moderate.wlz

20399_wholemount_weak.wlz

20399_wholemount_possible.wlz

20399_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20399_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

strong

regional

strong expression: see section 08 09 10 11

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 07 08 09 10

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 07 08 12 13

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 12 13 14 15 16 17 18 19 weak expression: see section 09

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11

pons ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

midbrain ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 14 15

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 13 14 15 16 17 18

ventral grey horn

strong

regional

strong expression: see section 06 weak expression: see section 07 08 09 10 11

spinal cord ventricular layer

strong

regional

strong expression: see section 07 08 09

dorsal root ganglion

weak

regional

weak expression: see section 06 08 09 10 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31926
Entity Detected:	Mt3, metallothionein 3 ( MGI:97173)
Sequence:	sense strand is shown >T31926 GTGGTTCCTGCACCTGCTCGGACAAATGCAAGTGCAAGGGCTGCAAATGCACGAACTGCAAGAAGAGCTG CTGCTCCTGCTGCCCTGCCGGATGTGAGAAGTGTGCCAAGGACTGTGTGTGCAAAGGTGAAGAGGGGGCC AAGGCAGAGGCCGAGAAATGCAGCTGCTGCCAGTGAGGACCCAGACCCTCCCACACAGCCTATGTAAATA GTGCTGGGTGTCCCTGGTGGGGCACAACTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6600430), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 61288. Forward Primer - name:061288_F_IRAW5_h10_Mt3, sequence:GTGGTTCCTGCACCTGCT; Reverse Primer - name:061288_R_SP6_IRAW5_h10_Mt3, sequence:ACAGTTGTGCCCCACCAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20396 same embryo
	EMAGE:20398 same embryo
	EMAGE:20397 same embryo
	EurExpress:euxassay_012667 same experiment
	MGI:4826479 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source