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EMAGE:204

Crabp1 cellular retinoic acid binding protein I ( MGI:88490)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:204 EMAGE:204
Fig 3 Ruberte et al, 1992 [PMID:1333403] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright. Fig 3 Ruberte et al, 1992 [PMID:1333403] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Serial transverse sections across the forelimb level at day 10.5. Corresponding bright and darkfield images are shown.Since plane of sections is slightly oblique, the left limb represent a more anterior plane of section than the right limb. Arrow in (C) points to AER. Ant, anterior; Post, posterior; L, left; R, right; D, dorsal; V, ventral; fp, floor plate; m, motoneurons; rp, roof plate.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
forelimb bud mesenchyme
detected detected
regionalMore abundant in distal regions, particularly in anterior regions; absent from more proximal central and dorsal mesenchyme.
neural tube lateral wall
detected detected
regionalTranscripts preferentially found in marginal and mantle layers.
cranial ganglion
detected detected
neural tube floor plate
not detected not detected
No transcripts in floor and roof plates of the neural tube
neural tube roof plate
not detected not detected
No transcripts in floor and roof plates of the neural tube
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:14729
Entity Detected:Crabp1, cellular retinoic acid binding protein I ( MGI:88490)
Sequence:sense strand is shown

>MGI:14729
CCAGCGTGCGACCGCTCCGCAGCAGAGGTGGGTGCCTGCCCCTGCCTTCGCGCCGCCGTACAGCCAACAC
CACTGCCACCATGCCCAACTTCGCCGGTACCTGGAAGATGCGCAGCAGCGAGAATTTCGACGAGCTCCTC
AAGGCGTTGGGTGTGAACGCCATGCTGAGGAAGGTGGCCGTGGCGGCTGCGTCTAAGCCGCACGTGGAGA
TCCGCCAAGACGGGGATCAGTTCTACATCAAGACATCCACTACTGTGCGCACCACGGAGATCAACTTCAA
GGTCGGAGAGGGCTTCGAGGAGGAGACAGTGGACGGACGCAAATGCAGGAGTTTACCCACGTGGGAGAAT
GAGAACAAGATTCACTGCACACAGACTCTTCTGGAGGGGGATGGCCCCAAAACTTACTGGACCCGAGAGC
TGGCCAACGATGAGCTCATCCTGACATTTGGCGCCGATGATGTGGTCTGCACGAGGATTTATGTCCGGGA
GTGAAAGGTGGCCAGCTTGTTCCTGCTTCATGACGATGCGAGTTCCCCTGAGGGGTATGCCGTGGCCCCA
CACTGCCAGTGGGTCTTTACTCCACACACCTCTCCCCCATGAATATTAGGCAACCCCATTTTCCCCATGA
CATTGTTGTAGTGTCCTCCCCTCAGGCTCTTGTTGCCTTGTGTACTGTGTACCCTTGTTTGGCATTTGCA
TGATGTACCAGTCATTAAACTGGTTGGCTGC
nt 1 - nt 731 of X15481.1
Notes:The probe used in this study by Ruberte et al., 1992 [PMID:1333403] is indicated as corresponding to the full length cDNA originally described by Stoner & Gudas, 1989 [PMID:2538228] (see Fig1B in the latter for sequence). The probe includes a poly(A) tail not included in the sequence link above. Probe length was "reduced to an average of 150 nucleotides by limited alkaline hydrolysis".
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Ruberte et al., 1992 [PMID:1333403] . Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1333403] Ruberte E, Friederich V, Morriss-Kay G, Chambon P 1992 Differential distribution patterns of CRABP I and CRABP II transcripts during mouse embryogenesis. Development (115):973-87
 [ PMID:2538228] Stoner CM, Gudas LJ 1989 Mouse cellular retinoic acid binding protein: cloning, complementary DNA sequence, and messenger RNA expression during the retinoic acid-induced differentiation of F9 wild type and RA-3-10 mutant teratocarcinoma cells. Cancer Res (49):1497-504
Links:MGI:2150737 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE