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EMAGE:20432

Tspan11 tetraspanin 11 ( MGI:1915748)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20432 EMAGE:20432 EMAGE:20432 EMAGE:20432 EMAGE:20432
"Pseudo-wholemount" of euxassay_012678. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012678_01 euxassay_012678_02 euxassay_012678_03 euxassay_012678_04
EMAGE:20432 EMAGE:20432 EMAGE:20432 EMAGE:20432 EMAGE:20432
euxassay_012678_05 euxassay_012678_06 euxassay_012678_07 euxassay_012678_08 euxassay_012678_09
EMAGE:20432 EMAGE:20432 EMAGE:20432 EMAGE:20432 EMAGE:20432
euxassay_012678_10 euxassay_012678_11 euxassay_012678_12 euxassay_012678_13 euxassay_012678_14
EMAGE:20432 EMAGE:20432 EMAGE:20432 EMAGE:20432 EMAGE:20432
euxassay_012678_15 euxassay_012678_16 euxassay_012678_17 euxassay_012678_18 euxassay_012678_19
EMAGE:20432 EMAGE:20432 EMAGE:20432
euxassay_012678_20 euxassay_012678_21 euxassay_012678_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20432Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20432_wholemount_strong.wlz
20432_wholemount_moderate.wlz
20432_wholemount_weak.wlz
20432_wholemount_possible.wlz
20432_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20432_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vibrissa
weak weak
regionalweak expression: see section 04 05 06 07 08 19 20 21 22
hypothalamus ventricular layer
weak weak
regionalweak expression: see section 13
diencephalon lateral wall ventricular layer
weak weak
regionalweak expression: see section 11 12 13
medulla oblongata floor plate
weak weak
regionalweak expression: see section 12
midbrain ventricular layer
strong strong
regionalstrong expression: see section 05 06 07 moderate expression: see section 09 13 14 15 16 weak expression: see section 08 10 11 12
spinal cord floor plate
weak weak
regionalweak expression: see section 09 10 11 12
nasal septum
weak weak
regionalweak expression: see section 11 12 13 14 15 16
mandible
moderate moderate
regionalmoderate expression: see section 09 10 11 12 16 17 18 19 weak expression: see section 06 07 08
maxilla
moderate moderate
regionalmoderate expression: see section 11 12 16 17
genital tubercle of male
moderate moderate
regionalmoderate expression: see section 09 11 weak expression: see section 10 12 13
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 21 22 weak expression: see section 20
clavicle
weak weak
regionalweak expression: see section 08 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32150
Entity Detected:Tspan11, tetraspanin 11 ( MGI:1915748)
Sequence:sense strand is shown

>T32150
AGGATGACTGGCTGCTGGCCCACCTGAAGTACCTGCTCTTCATCTTCAACTTCTTCTTCTGGGTAGGTGG
GGCAGCCGTCATGGCCGTGGGTATCTGGACACTGGTGGAGAAGAGCGGTTACCTCAGCATCTTGGCCTCC
AGCACGTTCGCTGCCTCTGCCTACATCCTCATTTTCGTTGGTGGTCTTGTCATGACAACTGGCTTCCTGG
GCTTTGGGGCCATCATCCGGGAGCAGAAGAGCTGCCTCTCCACATATTTCTGCCTGCTGCTGGTCATCTT
CCTTGTGGAGCTGGTGGCAGGGGTCCTGGCACATGTGTACTACCAGAGGCTGAGTGACGAGCTGAAGTGG
CACCTGAACAGCACCCTGACTGAGCACTACGGACAGCCCAGGGCCGCAGAGATCACAGCCTCGGTGGACC
GGCTGCAGCAGGACTTCAAATGCTGTGGCAGTAACAGCTCAGCCGACTGGCAGCACAGCGCGTACATCTT
GTCTCAGGAAGCCCTGGGTCGCCAGGTGCCCGACAGCTGCTGCAAGACAGTGGTGGCCCGCTGTGGTCAG
CGAGCCCACCCCTCCAACATCTACAAGGTAGAGGGAGGCTGTATGGCCAAGCTGGAACAGTTCGTGGCTG
ACCACCTGCTGCTCATGGGAGCGGTGGGTATTGGGGTAGCCTGTCTTCAGATCTGTGGGATGGTCCTCAC
CTGCTGCTTGCACCGAAGGCTCCAGCAACAATTTTATTAAAGGACACCCATGTACCCTGCTGACTGGCCC
ACCCAAGGGACCACATCTCCACTTCCTGAACCAGGCCTGCAAAGTCATCGCTGGGGCTGTGGACTCGCTC
CACCCTATGCAGAGGCCACCAAATGTCTGTGGTTGTGGTTCCTGTGCATCCCGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4218418), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60033. Forward Primer - name:060033_F_IRAV96_f02_1110014F12Rik, sequence:AGGATGACTGGCTGCTGG; Reverse Primer - name:060033_R_SP6_IRAV96_f02_1110014F12Rik, sequence:GTCGGGATGCACAGGAAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20430 same embryo
 EMAGE:20433 same embryo
 EMAGE:20429 same embryo
 EMAGE:20431 same embryo
 EMAGE:20428 same embryo
 EurExpress:euxassay_012678 same experiment
 MGI:4828958 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS