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EMAGE:20448

Ell elongation factor RNA polymerase II ( MGI:109377)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20448 EMAGE:20448 EMAGE:20448 EMAGE:20448 EMAGE:20448
"Pseudo-wholemount" of euxassay_012700. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012700_01 euxassay_012700_02 euxassay_012700_03 euxassay_012700_04
EMAGE:20448 EMAGE:20448 EMAGE:20448 EMAGE:20448 EMAGE:20448
euxassay_012700_05 euxassay_012700_06 euxassay_012700_07 euxassay_012700_08 euxassay_012700_09
EMAGE:20448 EMAGE:20448 EMAGE:20448 EMAGE:20448 EMAGE:20448
euxassay_012700_10 euxassay_012700_11 euxassay_012700_12 euxassay_012700_13 euxassay_012700_14
EMAGE:20448 EMAGE:20448 EMAGE:20448 EMAGE:20448 EMAGE:20448
euxassay_012700_15 euxassay_012700_16 euxassay_012700_17 euxassay_012700_18 euxassay_012700_19
EMAGE:20448 EMAGE:20448 EMAGE:20448 EMAGE:20448
euxassay_012700_20 euxassay_012700_21 euxassay_012700_22 euxassay_012700_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20448Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20448_wholemount_strong.wlz
20448_wholemount_moderate.wlz
20448_wholemount_weak.wlz
20448_wholemount_possible.wlz
20448_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20448_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31395
Entity Detected:Ell, elongation factor RNA polymerase II ( MGI:109377)
Sequence:sense strand is shown

>T31395
ATGGACGCTCTGCCTCACCCTCTCAGAAACGGCCTACAGACTTCATTGACCCCCTGGCCAGCAAGAAGCC
CCGGATCTCACATTTCACACAGCGAGCACAACCCACCCTCAATGGCAAACTGGGTGCCCCCAATGGCCAT
GAGACACTGCTGCCTGCTCCAGGACCCACCCCATCAGACACCCTCAGCTCTAGCCATCTGCCCCCACGGC
TGGAGCCCCCAAGGACCCACGACCCCCTAGCTGATGTCAGTAATGACCTAGGTCACAGTACCCAGGACTA
CAAGCACCAGGAAGCCACCCCAGCTCCAGCACCCCATCTTGGTCTTCCCCTGCTGACGGACTTTCCTCAG
GCTGAGCAACCTACTAGCTCCTCACACACCCACAGCCGACCCAAGAAGAAGTCCAAGAAGCACAAAGACA
AGGAGCGGCCCCCTGAAGAAAGGCCCCCCGCCCCACAGCCTGATGCACCTACTGCCCCTGCACTACCGCC
AGATGCCCCAGGTCTGAATGGAGCCTGTGACAATGAACCCACATCCTCGTCAGAGACCCCGGACTATTTG
CTGAAATACCCAGCCATCTCGTCGTCAGAGCAGCGTCAGAGCTACAAGAATGACTTCAATGCTGAGTACA
GCGAGTACCGCAGTCTGCACGCGAGGATCGAGCAGATCACCCGCAGGTTCACCCAGCTTGATGCACAGCT
CAGACAGCTGAGCCAGGGCTCTGATGAGTACGAGACAACCCGTGGGCAAATTCTTCAGGAATACAGGAAA
ATCAAAAAGACCAACACCAACTACAGCTGTGAGAAGCGACGCTGCGAATACCTGCACCGCAAGCTGGCGC
ACATTAAGAGGCTCATCGCAGAGTATGACCAGCGGCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4159557), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22616. Forward Primer - name:022616_F_IRAV55-58_K21_Ell, sequence:ATGGACGCTCTGCCTCAC; Reverse Primer - name:022616_R_SP6_IRAV55-58_K21_Ell, sequence:GCTGCCGCTGGTCATACT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20449 same embryo
 EMAGE:20451 same embryo
 EMAGE:20450 same embryo
 EMAGE:20446 same embryo
 EMAGE:20447 same embryo
 EurExpress:euxassay_012700 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS