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EMAGE:20452

Ythdc2 YTH domain containing 2 ( MGI:2448561)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20452 EMAGE:20452 EMAGE:20452 EMAGE:20452 EMAGE:20452
"Pseudo-wholemount" of euxassay_012731. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012731_01 euxassay_012731_02 euxassay_012731_03 euxassay_012731_04
EMAGE:20452 EMAGE:20452 EMAGE:20452 EMAGE:20452 EMAGE:20452
euxassay_012731_05 euxassay_012731_06 euxassay_012731_07 euxassay_012731_08 euxassay_012731_09
EMAGE:20452 EMAGE:20452 EMAGE:20452 EMAGE:20452 EMAGE:20452
euxassay_012731_10 euxassay_012731_11 euxassay_012731_12 euxassay_012731_13 euxassay_012731_14
EMAGE:20452 EMAGE:20452 EMAGE:20452 EMAGE:20452 EMAGE:20452
euxassay_012731_15 euxassay_012731_16 euxassay_012731_17 euxassay_012731_18 euxassay_012731_19
EMAGE:20452
euxassay_012731_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20452Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20452_wholemount_strong.wlz
20452_wholemount_moderate.wlz
20452_wholemount_weak.wlz
20452_wholemount_possible.wlz
20452_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20452_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38097
Entity Detected:Ythdc2, YTH domain containing 2 ( MGI:2448561)
Sequence:sense strand is shown

>T38097
AAGTGTTGTATGCCATGAGTGGATTTGAGGCATAGAGTATTCCTTCAGAATCATCATCTTCATATAGAGG
AAAAGATAATTTAAATTTTTCTAGTGGTTTTAAGGAAATTATTATATAATGTTTGTTAATTATCTGTTTC
AGATGAACAAAATAGCAATTTTGGTATACAACAGCACTTAATTTAATTTAATTTGAATGTAAACATTTCT
TTAAAATATTCAGTTAATGACTGTTGATGCCAAAGTCTTTTCTTTTTGCTCTTTCCAGACAGGGTTTCTT
TCTTTGTTTAGCCTCAACTGTCCAGGAACTAGCTCTGTAAGACCAGGCTGACCTCAAACTCACAGATATC
TGCCTGTTTGTTTCTGCCTCCTGAATACTGGAATTAAAGGCTTGCACCACCTCTGCCTGAGATGCCAAAC
TCTTATTGTTGACTTTTTGGTTGTGAGCCTATCCTTTAATGGCTGAGCCATCTCTCCAGCCCTAATGTTG
ACTTTTTAATTCAGGAAAGGCATAAATATATCAGTACATCTAAGAACCATTCTAGAAAAGTCGGTTCTTA
GGTTTATCATTAAAAAAACAACCAACACACTAAAATTTTAAATTATCTCTTACAGAACTTGTATACATAA
GGGCTATTCATTGTTATTTTTAGCCTGAAATATAGTAAAAAACAGGAGTGCTATTTTTCTCTGATTACAG
AATGAGATAAAAATCTCAAGAAAGGTAATTTCTCTTGACTTTTGATGAGATCAGTGCTTAATTTCATTCC
TAGTCCTGTCTCTTTTAACCTTATGTAAAATATACCCATTCTTTTTTTGCTTCCCATGAAGCAACCTTCA
CATAAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85801. Forward Primer - name:085801_F_cDNA_Ythdc2, sequence:AAGTGTTGTATGCCATGAGTGG; Reverse Primer - name:085801_N_SP6_cDNA_Ythdc2, sequence:GTTATGTGAAGGTTGCTTCATGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20457 same embryo
 EMAGE:20454 same embryo
 EMAGE:20455 same embryo
 EMAGE:20456 same embryo
 EMAGE:20453 same embryo
 EurExpress:euxassay_012731 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS