Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:20485

Madd MAP-kinase activating death domain ( MGI:2444672)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20485 EMAGE:20485 EMAGE:20485 EMAGE:20485 EMAGE:20485
"Pseudo-wholemount" of euxassay_012746. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012746_01 euxassay_012746_02 euxassay_012746_03 euxassay_012746_04
EMAGE:20485 EMAGE:20485 EMAGE:20485 EMAGE:20485 EMAGE:20485
euxassay_012746_05 euxassay_012746_06 euxassay_012746_07 euxassay_012746_08 euxassay_012746_09
EMAGE:20485 EMAGE:20485 EMAGE:20485 EMAGE:20485 EMAGE:20485
euxassay_012746_10 euxassay_012746_11 euxassay_012746_12 euxassay_012746_13 euxassay_012746_14
EMAGE:20485 EMAGE:20485 EMAGE:20485 EMAGE:20485 EMAGE:20485
euxassay_012746_15 euxassay_012746_16 euxassay_012746_17 euxassay_012746_18 euxassay_012746_19
EMAGE:20485 EMAGE:20485 EMAGE:20485 EMAGE:20485 EMAGE:20485
euxassay_012746_20 euxassay_012746_21 euxassay_012746_22 euxassay_012746_23 euxassay_012746_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20485Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20485_wholemount_strong.wlz
20485_wholemount_moderate.wlz
20485_wholemount_weak.wlz
20485_wholemount_possible.wlz
20485_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20485_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
facial vii ganglion
strong strong
regionalstrong expression: see section 05 06 07 19 20 21 22
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 07 08 18 19
trigeminal v ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 17 18 19 20 21 22
vagus x ganglion
strong strong
regionalstrong expression: see section 09 18 19
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 07 08 09 17 18 19 20
trigeminal v nerve
strong strong
regionalstrong expression: see section 10 17
spinal cord
strong strong
regionalstrong expression: see section 11 12 13 14 15 16
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 17 moderate expression: see section 11
cervical ganglion
strong strong
regionalstrong expression: see section 17 18 moderate expression: see section 09 10
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 13 14 15
dorsal root ganglion
strong strong
regionalstrong expression: see section 10 11 12 13 15 16 17 18 19
neural retina
strong strong
regionalstrong expression: see section 01 02 03 04 23 24
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 12 13 15 16 17 18 19
vomeronasal organ
strong strong
regionalstrong expression: see section 13 15
tongue
moderate moderate
spottedmoderate expression: see section 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31824
Entity Detected:Madd, MAP-kinase activating death domain ( MGI:2444672)
Sequence:sense strand is shown

>T31824
CAGGCCCTGACATTTGCTCTTCCAGATCCATCTCGATTCACCCTAGTAGATTTCCCTCTTCACCTTCCCT
TGGAACTTCTGGGTGTAGATGCTTGTCTTCAGGTGCTAACCTGCATCCTGTTGGAGCACAAGGTGGTGCT
GCAGTCTCGAGACTACAATGCACTCTCCATGTCTGTGATGGCGTTCGTGGCAATGATCTACCCCCTGGAG
TACATGTTCCCGGTCATCCCGCTGCTTCCCACCTGCATGGCCTCGGCAGAGCAGCTACTCTTGGCTCCAA
CCCCATACATCATTGGAGTTCCTGCCAGCTTCTTCCTCTACAAGCTAGATTTCAAAATGCCCGATGACGT
GTGGCTAGTGGATCTGGACAGCAATAGGGTGATTGCCCCCACCAATGCAGAAGTGCTACCCATCCTGCCA
GAACCAGAATCATTAGAGCTGAAAAAACACTTAAAGCAGGCCTTAGCCAGCATGAGTCTCAACACCCAGC
CCATTCTTAACCTGGAGAAATTCCACGAGGGCCAGGAGATCCCGCTTCTCTTGGGAAGGCCATCTAATGA
CCTTCAGTCCACACCTTCCACTGAATTCAACCCGCTCATTTATGGCAATGATGTAGATTCAGTCGACGTT
GCAACGAGAGTGGCCATGGTACGTTTCTTCAACTCTGCTAACGTGCTGCAGGGCTTTCAGATGCACACAC
GTACCCTGCGACTCTTCCCCCGGCCTGTGGTAGCTTTCCAAGCTGGCTCCTTTCTGGCCTCACGTCCCCG
GCAGACTCCCTTTGCTGAGAAACTGGCCAGGACCCAAGCTGTGGAGTATTTTGGAGAATGGATCCTGAAC
CCTTCTAACTATGCCTTCCAGCGGATTCACAACAATACGTTTGATCCGGCCCTTATTGGTGACAAGCCAA
AGTGGTACGCTCACCAGCTGCAGCCCATCCATTATC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5401819), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60044. Forward Primer - name:060044_F_IRAV99_h11_Madd, sequence:CAGGCCCTGACATTTGCT; Reverse Primer - name:060044_R_SP6_IRAV99_h11_Madd, sequence:CGATAATGGATGGGCTGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20483 same embryo
 EMAGE:20482 same embryo
 EMAGE:20484 same embryo
 EMAGE:20487 same embryo
 EMAGE:20486 same embryo
 EurExpress:euxassay_012746 same experiment
 MGI:4826055 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS