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EMAGE:20491

Zkscan1 zinc finger with KRAB and SCAN domains 1 ( MGI:1921820)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20491 EMAGE:20491 EMAGE:20491 EMAGE:20491 EMAGE:20491
"Pseudo-wholemount" of euxassay_012753. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012753_01 euxassay_012753_02 euxassay_012753_03 euxassay_012753_04
EMAGE:20491 EMAGE:20491 EMAGE:20491 EMAGE:20491 EMAGE:20491
euxassay_012753_05 euxassay_012753_06 euxassay_012753_07 euxassay_012753_08 euxassay_012753_09
EMAGE:20491 EMAGE:20491 EMAGE:20491 EMAGE:20491 EMAGE:20491
euxassay_012753_10 euxassay_012753_11 euxassay_012753_12 euxassay_012753_13 euxassay_012753_14
EMAGE:20491 EMAGE:20491 EMAGE:20491 EMAGE:20491 EMAGE:20491
euxassay_012753_15 euxassay_012753_16 euxassay_012753_17 euxassay_012753_18 euxassay_012753_19
EMAGE:20491 EMAGE:20491 EMAGE:20491
euxassay_012753_20 euxassay_012753_21 euxassay_012753_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20491Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20491_wholemount_strong.wlz
20491_wholemount_moderate.wlz
20491_wholemount_weak.wlz
20491_wholemount_possible.wlz
20491_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20491_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 17
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 16
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 16 17 18 19
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 07
spinal cord
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 11 15
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 15 16 17 18
metanephros
weak weak
regionalweak expression: see section 07 08 09 10 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38217
Entity Detected:Zkscan1, zinc finger with KRAB and SCAN domains 1 ( MGI:1921820)
Sequence:sense strand is shown

>T38217
GCCAGAGAGAGAACGTCAGAATACAGCCCAGCCTCTCTGGATGCCTTTGGGGCATTCCTGAAAAGTTGTG
TGTAAAGCAGGAGTGTCTCGTCAAGCCATTTCCCCCCTTTTGTTTCTGGAATTACCTATGTACCCATCGA
GGGAAAAGTAATAGCACCATCGAATTGTTTGGGAAGTCACACTTCAGGGTCTCTAACAGTTACATCAACC
ATGTCCTCCCTTCAGGAAGTCTGTAGACATGCAATTCTTAAAGTCCGTGTAAGGGAAAGCTGGCCGTGTG
GATATTCCTGCACTCAAGATAAAAGCATGCACAGTAAACTGTCAAGCTTTCTAGTCATGGGTATACCTTT
AAGGTTTGCTTCCCAGCAACCACACCATATAGTTGATGAGTCAAGATCAGCTCTGTGAATATTATACTGG
TTAAGCCACTTGCTGCAAACAAACCCTAATTAGACAACTTGTATTTCACAATGAAGAGAGATAGTTAATA
CAAAAACTACATTGGGACATGCTGCTTGAGCAAGTTGTAAATCCAGAAGGTTCTATATCTGATCACTGGT
AGCCTTCCCAAGCTATAGAAATGTAGGACTGTGTTGGTCAGAATCAAACCAAAGATTTCTATCTCTTTCT
GAAAGAGGGCATATGCACCAGTTTATAGTTCCAAGGACTGTAACAAATGTGGATGGGCCTGCCCCCATCA
GTGAGATCAGTCCTACTGAAATAGCAACAATTCAAGATGCTTCTCTTCCTCTTCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 147703. Forward Primer - name:147703_F_cDNA_9130423L19Rik, sequence:GCCAGAGAGAGAACGTCAGAAT; Reverse Primer - name:147703_N_SP6_cDNA_9130423L19Rik, sequence:TGAAGAGGAAGAGAAGCATCTTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20490 same embryo
 EMAGE:20492 same embryo
 EMAGE:20488 same embryo
 EMAGE:20489 same embryo
 EurExpress:euxassay_012753 same experiment
 MGI:4829357 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS