EMAGE mouse gene expression spatial database

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EMAGE:20510

Ift80 intraflagellar transport 80 homolog (Chlamydomonas) ( MGI:1915509)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002024. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002024_01	euxassay_002024_02	euxassay_002024_03	euxassay_002024_04

euxassay_002024_05	euxassay_002024_06	euxassay_002024_07	euxassay_002024_08	euxassay_002024_09

euxassay_002024_10	euxassay_002024_11	euxassay_002024_12	euxassay_002024_13	euxassay_002024_14

euxassay_002024_15	euxassay_002024_16	euxassay_002024_17	euxassay_002024_18	euxassay_002024_19

euxassay_002024_20	euxassay_002024_21	euxassay_002024_22	euxassay_002024_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20510_wholemount_strong.wlz

20510_wholemount_moderate.wlz

20510_wholemount_weak.wlz

20510_wholemount_possible.wlz

20510_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20510_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

weak

homogeneous

weak expression: see section 10 11 12 13

diencephalon lateral wall ventricular layer

weak

homogeneous

weak expression: see section 11 12 13

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 08 09 14 15

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain ventricular layer

weak

homogeneous

weak expression: see section 07 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2506
Entity Detected:	Ift80, intraflagellar transport 80 homolog (Chlamydomonas) ( MGI:1915509)
Sequence:	sense strand is shown >T2506 ATTGTAAAGTATCTTCCATACTTTATGAAACAAAAATACAGGAGAAAGGGTTTGGGGAAAATGGTACAAT GTAGATGTATATCGAAGTTTATAGTTCACATAACAAACGCTGGGAACAGCTATGGTATCCATCAGCTACA ATTAGATAAAACCACGTGGTATTCACCGGCAGTGATTAGTCTCTCATAGAAGGGTTGAGCTGTGGCACAT GCCGCAACGTGGATGAGCCTAGGAGAACTTCCACTACGTGAAGTAGGTTAGACATGGCACAGACGTCAGT CCTGCGGGGCTCACACAAGGCACTCGGATTAAGTAAGTTCTTCTGGTCAGAAAAGAGGCAAGAGGGACAG GCCCTGGAGGAGGGAATTCATTGTGTAACGCTGGCCATTGCTGCTTGGGGTGAGAGCCCTAGAAATGGAG AGTGGTGATGGTTGTATAGCATCGTGGGTGTTGTGAACTCCGTTGTCCTCTTTAAAAGGATTAAAATGAT AAATTTTCTTTCCAGTTGTTTTTTTTTTTAAACCATATTGAAAAAGTAG
Notes:	The probe template was PCR amplified from IMAGE:1265367 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1265367 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20511 same embryo
	EMAGE:20514 same embryo
	EMAGE:20513 same embryo
	EMAGE:20512 same embryo
	EurExpress:euxassay_002024 same experiment
	MGI:4825532 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source