EMAGE mouse gene expression spatial database

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EMAGE:20511

Asb2 ankyrin repeat and SOCS box-containing 2 ( MGI:1929743)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001878. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001878_01	euxassay_001878_02	euxassay_001878_03	euxassay_001878_04

euxassay_001878_05	euxassay_001878_06	euxassay_001878_07	euxassay_001878_08	euxassay_001878_09

euxassay_001878_10	euxassay_001878_11	euxassay_001878_12	euxassay_001878_13	euxassay_001878_14

euxassay_001878_15	euxassay_001878_16	euxassay_001878_17	euxassay_001878_18	euxassay_001878_19

euxassay_001878_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20511_wholemount_strong.wlz

20511_wholemount_moderate.wlz

20511_wholemount_weak.wlz

20511_wholemount_possible.wlz

20511_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20511_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 04 15 16 17 18 19

head mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 14 16 17 18 19 20

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

foregut-midgut junction

strong

regional

strong expression: see section 09 10 11 12

heart ventricle

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

esophagus

strong

regional

strong expression: see section 08 09 10 11 12 13

tongue

strong

regional

strong expression: see section 09 10 11 12 13 14

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08

hindgut

strong

regional

strong expression: see section 10 11 12 13 14

midgut

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15

bladder

moderate

regional

moderate expression: see section 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3471
Entity Detected:	Asb2, ankyrin repeat and SOCS box-containing 2 ( MGI:1929743)
Sequence:	sense strand is shown >T3471 TGGCCTCGAGCCAGATTCGGCACGAGGCTGCCGGCTGCGGGTTCGGAAGGCCATAGGAAAATACCGGATA AAACTCCTGGACACACTGCCGCTTCCCGGCAGGCTAATCAGATACCTGAAATATGAGAATACACAGTAAC CAGCCTGGAGAGGAGATGTGGCCTTCAGACTGTTTCCGGGACGCCCCAGGTGGCCTGCATCCAGGACCCC CTGGGGTCAGAACAGGTGTGACCTTGCTGGTTCTTTGCTGGAGCTTCACCCAAAGTGAGAACCTGATGTG GGGAGTGGACGTGGAACCTCTGCTTTCACACTGTCAGCGGATCGCAGACCCGCTCTGCTTCTGGCCATAG CCAGAGACCTTCAACCTGGGGCCAGGGGAGAGCTGGTCTGGGCAAGGTGGCCCAGGCAGGAATCCTGGCC TTAAGCTGGAGAACTTGTAGGAATCCCTCACTGGACCCTCAGCTTTCAGGCTGCGAGGGAGACGCCCAGC CCAAGTATTTTATTTCACTGAGCACAATAAACGTTGTATACAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:331907 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:331907 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20514 same embryo
	EMAGE:20513 same embryo
	EMAGE:20512 same embryo
	EMAGE:20510 same embryo
	EurExpress:euxassay_001878 same experiment
	MGI:4823268 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source