EMAGE mouse gene expression spatial database

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EMAGE:20557

Acaa2 acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) ( MGI:1098623)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012769. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012769_01	euxassay_012769_02	euxassay_012769_03	euxassay_012769_04

euxassay_012769_05	euxassay_012769_06	euxassay_012769_07	euxassay_012769_08	euxassay_012769_09

euxassay_012769_10	euxassay_012769_11	euxassay_012769_12	euxassay_012769_13	euxassay_012769_14

euxassay_012769_15	euxassay_012769_16	euxassay_012769_17	euxassay_012769_18	euxassay_012769_19

euxassay_012769_20	euxassay_012769_21	euxassay_012769_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20557_wholemount_strong.wlz

20557_wholemount_moderate.wlz

20557_wholemount_weak.wlz

20557_wholemount_possible.wlz

20557_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20557_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vibrissa

strong

regional

strong expression: see section 03

diencephalon roof plate

strong

regional

strong expression: see section 13

choroid invagination

strong

regional

strong expression: see section 07 08 09 10 11 16 17 18 19 20

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

tongue epithelium

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14

oral epithelium

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

bladder

strong

regional

strong expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38273
Entity Detected:	Acaa2, acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) ( MGI:1098623)
Sequence:	sense strand is shown >T38273 AGTCCCCCTACTGTGTCAGAAATGTGCGCTTCGGAACCAAATTTGGATTAGATCTCAAGCTGGAAGATAC TTTGTGGGCAGGATTAACGGATCAACATGTTAAGCTGCCCATGGGAATGACTGCAGAGAACCTTGCTGCA AAATACAACATAAGCAGAGAAGACTGTGACAGATACGCCTTGCAGTCTCAGCAGAGGTGGAAAGCTGCTA ACGAGGCTGGCTACTTCAATGAGGAGATGGCACCCATTGAGGTGAAGACGAAGAAAGGCAAACAGACCAT GCAAGTGGACGAGCACGCTCGACCCCAAACCACCCTGGAGCAACTGCAGAAGCTCCCGTCCGTGTTCAAG AAAGACGGGACAGTCACAGCAGGGAACGCCTCGGGGGTGTCTGACGGTGCTGGGGCCGTCATCATAGCCA GCGAAGATGCTGTCAAAAAACATAACTTCACGCCCCTGGCCAGAGTCGTGGGCTACTTCGTGTCCGGATG CGATCCTACTATCATGGGTATTGGTCCAGTCCCTGCTATCAATGGAGCATTGAAGAAAGCTGGGCTGAGT CTTAAGGACATGGATTTGATAGACGTGAACGAAGCTTTTGCCCCTCAGTTCTTGTCTGTTCAGAAGGCCC TGGATCTTGACCCCAGCAAAACCAATGTGAGTGGAGGCGCCATTGCCCTGGGTCACCCGCTGGGAGGATC TGGCTCCAGAATC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 142865. Forward Primer - name:142865_F_cDNA_Acaa2, sequence:AGTCCCCCTACTGTGTCAGAAA; Reverse Primer - name:142865_N_SP6_cDNA_Acaa2, sequence:GATTCTGGAGCCAGATCCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20558 same embryo
	EMAGE:20556 same embryo
	EurExpress:euxassay_012769 same experiment
	MGI:4822895 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source