EMAGE mouse gene expression spatial database

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EMAGE:20605

Svep1 sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1 ( MGI:1928849)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013843. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013843_01	euxassay_013843_02	euxassay_013843_03	euxassay_013843_04

euxassay_013843_05	euxassay_013843_06	euxassay_013843_07	euxassay_013843_08	euxassay_013843_09

euxassay_013843_10	euxassay_013843_11	euxassay_013843_12	euxassay_013843_13	euxassay_013843_14

euxassay_013843_15	euxassay_013843_16	euxassay_013843_17	euxassay_013843_18	euxassay_013843_19

euxassay_013843_20	euxassay_013843_21	euxassay_013843_22	euxassay_013843_23	euxassay_013843_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20605_wholemount_strong.wlz

20605_wholemount_moderate.wlz

20605_wholemount_weak.wlz

20605_wholemount_possible.wlz

20605_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20605_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

viscerocranium

moderate

regional

Expression in the turbinate bone.

esophagus

weak

regional

weak expression: see section 13 14

stomach

weak

regional

weak expression: see section 01 02 03 04

hindgut

weak

regional

weak expression: see section 12

midgut

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15

lower lip

moderate

regional

moderate expression: see section 08 09 11 12 13 16 17 18 19 20 weak expression: see section 14 15

upper lip

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 20

bladder

weak

regional

weak expression: see section 11 12 13 14 15

male reproductive system

weak

regional

weak expression: see section 11 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38801
Entity Detected:	Svep1, sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1 ( MGI:1928849)
Sequence:	sense strand is shown >T38801 AACTCAGGTGTCTGGAAGGGTATGTGATGGATTCGGATACAGATACATTCACCTGCCAGCAAGATGGCCA TTGGGTCCCTGAAAGAATCACCTGCAGTCCTAAAAAATGCCCTGTGCCATCCAACATGACACGCATACGT TTTCACGGAGATGACTTCCAGGTGAACAGACAAGTTTCTGTGTCATGTGCAGAAGGGTTTACCCACGAAG GAGTGAACTGGTCAACATGCCAGCCCGACGGTACATGGGAGCCACCATTTTCTGATGAATCCTGTATCCC AGTTGTTTGTGGGCATCCTGAAAGCCCAGCGCATGGCTCCGTGGTTGGCAATAAGCACAGCTTTGGAAGC ACCATTGTTTACCAGTGTGACCCTGGCTACAAATTAGAGGGGAACAGGGAACGAATCTGCCAGGAGAACA GACAGTGGAGTGGAGAGGTGGCAGTGTGCAGAGAGAACAGATGTGAGACTCCAGCTGAGTTTCCCAATGG GAAGGCTGTCTTGGAAAACACCACATCTGGACCCAGCCTTCTGTTTTCCTGTCACAGAGGCTACACCCTG GAAGGGTCCCCCGAGGCACACTGCACTGCAAATGGAACCTGGAATCACCTGACTCCCCTCTGCAAACCAA ATCCATGCCCTGTCCCTTTTGTGATTCCTGAGAACGCCGTCCTTTCTGAAAAAGAGTTTTATGTCGACCA GAATGTATCTATCAAGTGCAGGGAAGGCTTCCTGCTCAAAGGCAATGGTGTCATCACGTGCAGCCCTGAC GAGACATGGACGCACACCAATGCCAGATGTGAAAAAATCTCCTGTGGTCCTCCAAGTCACGTAGAGAATG CAATTGCTCGAGGAGTGTATTACCAGTATGGGGACATGATCACCTACTCCTGTTACAGTGGCTACATGCT AGAAGGTTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 235847. Forward Primer - name:165002_F_cDNA_Polydom, sequence:AACTCAGGTGTCTGGAAGGGTA; Reverse Primer - name:165002_N_SP6_cDNA_Polydom, sequence:GGAACCTTCTAGCATGTAGCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20604 same embryo
	EMAGE:20602 same embryo
	EMAGE:20601 same embryo
	EMAGE:20603 same embryo
	EMAGE:20600 same embryo
	EurExpress:euxassay_013843 same experiment
	MGI:4828547 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source