EMAGE mouse gene expression spatial database

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EMAGE:20620

Snhg11 small nucleolar RNA host gene 11 ( MGI:2441845)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014130. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014130_01	euxassay_014130_02	euxassay_014130_03	euxassay_014130_04

euxassay_014130_05	euxassay_014130_06	euxassay_014130_07	euxassay_014130_08	euxassay_014130_09

euxassay_014130_10	euxassay_014130_11	euxassay_014130_12	euxassay_014130_13	euxassay_014130_14

euxassay_014130_15	euxassay_014130_16	euxassay_014130_17	euxassay_014130_18	euxassay_014130_19

euxassay_014130_20	euxassay_014130_21	euxassay_014130_22	euxassay_014130_23	euxassay_014130_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20620_wholemount_strong.wlz

20620_wholemount_moderate.wlz

20620_wholemount_weak.wlz

20620_wholemount_possible.wlz

20620_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20620_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 12 13 14 16 17 18 19

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22 23

rest of cerebellum mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pons mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 06 07 08 21 22 23 24

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 09 21 22

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 19 20 21 22 23 24

vagus x ganglion

strong

regional

strong expression: see section 09 10 20 21

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 08 09 10 20 21

trigeminal v nerve

strong

regional

strong expression: see section 11 19

spinal cord mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22

neural retina

strong

regional

strong expression: see section 02 03 04 05

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40390
Entity Detected:	Snhg11, small nucleolar RNA host gene 11 ( MGI:2441845)
Sequence:	sense strand is shown >T40390 GCCAAGGACTGAAAAACAGAAGTGTAGTCGATAGGAGTTAGATTACTTGAAAGTTTCCACCTAAGATTTT TTTGGTACAGCCTGAGAATAGGTGGGATGTAGTAAGGAATAGAACGGTCCAGAAAGTTCTTCTTCCATGG AAATGTTATTCTTCGATACTGTTCACATGTTTGTTTTGTTTGCAGTGTCAACCGGACATGTTCTTTCACA CTCTCGTGGGACAACCCAGTTGTTTCCCACTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 76952. Forward Primer - name:076952_F_cDNA_E130013N09Rik, sequence:GCCAAGGACTGAAAAACAGAAG; Reverse Primer - name:076952_N_SP6_cDNA_E130013N09Rik, sequence:GAAGTGGGAAACAACTGGGTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828349 same experiment
	EurExpress:euxassay_014130 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source