EMAGE mouse gene expression spatial database

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EMAGE:20632

Hnrnph3 heterogeneous nuclear ribonucleoprotein H3 ( MGI:1926462)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013902. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013902_01	euxassay_013902_02	euxassay_013902_03	euxassay_013902_04

euxassay_013902_05	euxassay_013902_06	euxassay_013902_07	euxassay_013902_08	euxassay_013902_09

euxassay_013902_10	euxassay_013902_11	euxassay_013902_12	euxassay_013902_13	euxassay_013902_14

euxassay_013902_15	euxassay_013902_16	euxassay_013902_17	euxassay_013902_18	euxassay_013902_19

euxassay_013902_20	euxassay_013902_21	euxassay_013902_22	euxassay_013902_23	euxassay_013902_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20632_wholemount_strong.wlz

20632_wholemount_moderate.wlz

20632_wholemount_weak.wlz

20632_wholemount_possible.wlz

20632_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20632_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 13 14 15 16

telencephalon ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38406
Entity Detected:	Hnrnph3, heterogeneous nuclear ribonucleoprotein H3 ( MGI:1926462)
Sequence:	sense strand is shown >T38406 GGAGGCAGTGGAGGTTACTATGGGCAAGGTGGAATGAGTGGAGGTGGATGGCGTGGGATGTACTGACAAC ACCACCCCAACGTACACGTCCTAACACACCTGCTCTACTAGACTTTCTTACAGATTTCATTTCTTTTGTA TTTCAAGAACTTTATAACGACTGAAGGAATGTGTTATATTATTTGGTAAAGCAACAGACTGCGATGGGAA AATCTGTTTTCTGTAGGTTGTTTCATTTGTTGCATACTTTGACTTAAATAAATTTTTATATTCAAACCAC TGATGTTGCTACTTTTTATATATAGGTACTCCTAAAGATGTGCTGCCTTCATAAGATTTGGGATAATGTA TCTTACTGTTAGCATAGTGCAGTGATAGAGGGCAAGACAGCGAGTCTTAAGTAGGCATCTAGGGTAGCAC TACCTTTCACCCCTGGACATACTGTAAATAAAAAGCCTAAAGATACGTTCAGGTGGTTTCAGGAACATAA GCTAATTCTGTATTCTTTTCGGTCTCTTTTATCAAGCATAGCTCTGAATAATTCAAGACGTATCTGGATT CTAAATATTCACAAATTGTATTATGAATGGGGGTAGGGGTATAAAACCCTCATAGCACTTGACTAAGATA CCAAAAATGGTTCCCAAAACTTGAGTTTGTTGGAGTAGTTTGAAATTCTTAATAATGACAGAAAAACTCT GTAAATACACGGGCATTTACTATAATGGGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 109607. Forward Primer - name:109607_F_cDNA_LOC432467, sequence:GGAGGCAGTGGAGGTTACTATG; Reverse Primer - name:109607_N_SP6_cDNA_LOC432467, sequence:ACCCATTATAGTAAATGCCCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20635 same embryo
	EMAGE:20634 same embryo
	EMAGE:20633 same embryo
	EurExpress:euxassay_013902 same experiment
	MGI:4825404 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source