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EMAGE:20664

Tgm7 transglutaminase 7 ( MGI:2151164)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20664 EMAGE:20664 EMAGE:20664 EMAGE:20664 EMAGE:20664
"Pseudo-wholemount" of euxassay_014048. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014048_01 euxassay_014048_02 euxassay_014048_03 euxassay_014048_04
EMAGE:20664 EMAGE:20664 EMAGE:20664 EMAGE:20664 EMAGE:20664
euxassay_014048_05 euxassay_014048_06 euxassay_014048_07 euxassay_014048_08 euxassay_014048_09
EMAGE:20664 EMAGE:20664 EMAGE:20664 EMAGE:20664 EMAGE:20664
euxassay_014048_10 euxassay_014048_11 euxassay_014048_12 euxassay_014048_13 euxassay_014048_14
EMAGE:20664 EMAGE:20664 EMAGE:20664 EMAGE:20664 EMAGE:20664
euxassay_014048_15 euxassay_014048_16 euxassay_014048_17 euxassay_014048_18 euxassay_014048_19
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euxassay_014048_20 euxassay_014048_21 euxassay_014048_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20664Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20664_wholemount_strong.wlz
20664_wholemount_moderate.wlz
20664_wholemount_weak.wlz
20664_wholemount_possible.wlz
20664_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20664_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
pancreas
strong strong
spottedstrong expression: see section 08 09 13 moderate expression: see section 10 11 12 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39763
Entity Detected:Tgm7, transglutaminase 7 ( MGI:2151164)
Sequence:sense strand is shown

>T39763
GGCCCATAACACCAGTTACATTGGGAAAGCAATTAGCACCAAGATGGTAGGGTCAGATGAGCGCCTGGAC
ATCACCAGTTCCTACAAGTATCCAGAAGGATCCTCTGAGGAGCGGTCTGCATTCATTAAGGCTTCCTGGA
AATTGCTGGAACCGAGAAGGAACGCTTCATCTCTCCTGGATCTCCTAGGGTCTGAGAACTTAAAGGCTAA
GCCTGTGAGGCTGCAACTCCACCTGGTTAGGGCACCCATGTGGGGCCAGGACCTGCCGCTCATGCTATAT
GTCCAGAGGGTGCCAGTCAGAGACCATGTTCAGGGCTCTGTCGGACTGACGGTGCGCTTTTGTGCACAGG
CCCTCCTGCATGGGGGTGGAACTCGGAAACCGTTCTGGAGACACATAGTACACTTGAATGTGGATTTTGA
GAAGGAGCTACAGTGGCCGTTCCTGCTGCCCTACAGCAGTTACAGGGACAAGCTGACAGATGAAAGGCTG
ATCCGTGTGGCTGGCATCGCTGAGGTTGAAGAGACAGGGCAGTTCATTCTGGTCCTAAAAGATTTCTCTC
TGGAGCCTCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79818. Forward Primer - name:079818_F_cDNA_Lcmt2, sequence:GGCCCATAACACCAGTTACATT; Reverse Primer - name:079818_N_SP6_cDNA_Lcmt2, sequence:GGGAGGCTCCAGAGAGAAATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20661 same embryo
 EMAGE:20662 same embryo
 EMAGE:20663 same embryo
 EMAGE:20666 same embryo
 EMAGE:20665 same embryo
 EurExpress:euxassay_014048 same experiment
 MGI:4828687 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS