EMAGE mouse gene expression spatial database

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EMAGE:20665

Sh2d4b SH2 domain containing 4B ( MGI:1925182)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014198. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014198_01	euxassay_014198_02	euxassay_014198_03	euxassay_014198_04

euxassay_014198_05	euxassay_014198_06	euxassay_014198_07	euxassay_014198_08	euxassay_014198_09

euxassay_014198_10	euxassay_014198_11	euxassay_014198_12	euxassay_014198_13	euxassay_014198_14

euxassay_014198_15	euxassay_014198_16	euxassay_014198_17	euxassay_014198_18	euxassay_014198_19

euxassay_014198_20	euxassay_014198_21	euxassay_014198_22	euxassay_014198_23	euxassay_014198_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20665_wholemount_strong.wlz

20665_wholemount_moderate.wlz

20665_wholemount_weak.wlz

20665_wholemount_possible.wlz

20665_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20665_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15

thalamus mantle layer

moderate

regional

moderate expression: see section 07 08 15 16 weak expression: see section 09 14

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 17 18 19 20 21 22 23

heart ventricle

moderate

spotted

moderate expression: see section 09 10 11 weak expression: see section 07 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39745
Entity Detected:	Sh2d4b, SH2 domain containing 4B ( MGI:1925182)
Sequence:	sense strand is shown >T39745 TCTGTCATGGCTGTGCTAAGTTGAATTCCTAGGGTGAATGGGTCATATTTTAATAACCCAAAGCAAATCC AGTGCCGAACCTCCCACTTAATGTAGGGCCAGCACTCAATAAGTCTTTGTTGAATGAATGCCCTCCTAAA AAACTCCTAACACTGATGGTTTCTAGCAAATTACTTTGTATATTACTTCATAAAGTCACAGAGATAGCAG ACCAGGCTTGTGACTAACTTACATGGAAGACACACATGTTATATCTTTCCTAGTATTCATGAACTTGTTG ATATTCTCATGGGCATTGCTCAAGGTTGTTATGTTTGACTTTCAGTATGGGATATTGGTGTTGTGCTGGC TGCTTGGTTTGACTACTTCTACCTCTCTCTTATGTCTCATCTTACATGTGGCTCTGGTTTTGCAAATTGA GGTAATGATGTCATGTAAATGCGCAGAATATCTGGAATGGATTATAAACCCAAGGATAGTGTTTAGATAC TTGATGAGGCAGGTCTTTGCTCTCTAGAATGTTCTCTGTGTGCTCTCTACTTAAAGGCGTAGCTATGCTT TGATTTTAATTCTTCCACCTCCTTAATTTGAACATGTTCTTCATATTAATGAGAGTAACCTCCCTGTAAC TCAGTAACTCTCAAGGATGGAGTCTTCCTTAGGGTTTTATTGTTGCAAAGAGACACCATGAACACAGCCA CTCATAAAATAAAATATTTAATTGGGGACTAGCTTATGGCTTCAGAGGTTCAGTCAATTGTCATTATGAT GGGAAGCATGGCAGCTTGTAAGCAGACATGGTTCAGGAGAAGGAGATAAGAGTTCCATACCTTGATCTGA AGGCAACTGGGAGAGAGACTGTCTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70487. Forward Primer - name:070487_F_cDNA_D030001E08, sequence:TCTGTCATGGCTGTGCTAAGTT; Reverse Primer - name:070487_N_SP6_cDNA_D030001E08, sequence:GAAGACAGTCTCTCTCCCAGTTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20661 same embryo
	EMAGE:20662 same embryo
	EMAGE:20663 same embryo
	EMAGE:20666 same embryo
	EMAGE:20664 same embryo
	EurExpress:euxassay_014198 same experiment
	MGI:4828034 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source